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ProteomicsMS analysis of BCG and recombinant BCG "70C-RBD"
BCG and recombinant BCG "70C-RBD" were cultured in Sauton media. Culture filtrate and cells lysate were separated by SDS-PAGE. The bands at 40kDa and 60kDa were excised and proteins were reduced and alkylated. In-gel digestion (Trypsin) was performed. The samples were analyzed by TripleTOF5600+ conj...
ORGANISM(S): Cellular Organisms 
2023-12-07 | PXD046929 | JPOST Repository
ProteomicsEpitope mapping of SARS-CoV 2 RBDs by hydroxyl radical protein footprinting reveals the importance of including negative antibody controls.
The focus of the present investigation was epitope mapping of a mouse monoclonal antibody to the receptor binding domain of two SARS-CoV-2 spike protein variants (Wild-Type and variant B.1.617.2). The experimental setup utilizing multiple controls allowed for validation of false positive binding on ...
ORGANISM(S): Mus musculus (Mouse) Severe acute respiratory syndrome coronavirus 2 
2025-02-21 | PXD046423 | Pride
Rbd alpha Fenton chemistry Covid-19 Mass spectrometry Rbd delta 
ProteomicsDifferential Proteomic Landscape of Plasma Neuron-Derived Extracellular Vesicles in Parkinson’s Disease with and without RBD
Parkinson’s disease (PD) is increasingly recognized as a heterogeneous disorder, with rapid eye movement sleep behavior disorder (RBD) serving as a key prodromal marker for a distinct, aggressive subtype. This study investigates the plasma neuron-derived extracellular vesicle (EV) proteome to deline...
ORGANISM(S): Homo sapiens (Human) 
2026-08-27 | PXD076025 | Pride
Neuron-derived extracellular vesicle Α-synuclein Lipid metabolism Hetrogenity L1cam Rapid eye movement sleep behavior disorder (rbd) Proteomics Extracellular matrix remodeling Subtype stratification Precision medicine Parkinson’s disease Biomarkers 
TranscriptomicsPeripheral Blood Mononuclear Cell Electrophysiology as a Marker for Immune Response to Exposure and Vaccination
Comparison of cell stimulated with SARS-CoV-2 RBD, peptide mix or control. Samples from 3 donors post second vaccine dose.
ORGANISM(S): Homo sapiens 
2025-04-01 | E-MTAB-14606 | biostudies-arrayexpress
UnknownGWAS by Subtraction to Disentangle RBD Genetic Background from α-Synucleinopathies.
Not available
S-EPMC12026788 | biostudies-literature
TranscriptomicsGene expression profiling of human pancreatic cancer cells treated with RRSP-RBD-TAT and RRSP-TAT compared with untreated controls
Human pancreatic cancer cells (PANC-1) were treated with a protein-based pan-RAS inhibitor (RRSP-RBD-TAT), a control construct (RRSP-TAT), or vehicle control. After 48 hours of treatment, total RNA was extracted and subjected to gene expression profiling using Agilent SurePrint G3 Human GE 8x60K mic...
ORGANISM(S): Homo sapiens 
2026-05-02 | E-MTAB-16847 | biostudies-arrayexpress
ProteomicsOnline Hydrophilic Interaction Chromatography (HILIC) Enhanced Top-Down Mass Spectrometry Characterization of SARS-CoV-2 Spike Receptor Binding Domain
Online LCMS intact glycoform separation of SARS-CoV-2 spike receptor binding domain proteins expressed in HEK-293 cells from the vendors Sino Biological or RayBiotech. C2 RPLC, CZE, and HILIC separation methods are compared as well as top-down and bottom-up analysis to define RBD glycosylation patte...
ORGANISM(S): Sars Coronavirus (ncbitaxon:227859) Homo Sapiens (ncbitaxon:9606) 
2021-12-21 | MSV000088601 | MassIVE
ProteomicsStructural dynamics in the evolution of SARS-CoV-2 spike glycoprotein: HDX-MS analysis of the spike of the original Wuhan isolate, G614 spike mutant, and the isolated ancestral RBD - in apo state and in complex with the ACE2 receptor
The spike glycoprotein of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates binding to the ACE2 receptor and subsequent membrane fusion. It exists in a range of conformations, including a closed state unable to bind the ACE2 receptor, and an open state that does so but displa...
ORGANISM(S): Homo sapiens (Human) 
2023-03-15 | PXD039760 | Pride
Sars-cov-2 Spike glycoprotein Hdx-ms 
ProteomicsStructural O-Glycoform Heterogeneity of the SARS-CoV-2 Spike Protein Receptor-Binding Domain Revealed by Top-Down Mass Spectrometry
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes an extensively glycosylated surface spike (S) protein to mediate host cell entry and the S protein glycosylation plays key roles in altering viral binding/function and infectivity. However, the molecular structures and glycan hete...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-07-27 | MSV000087891 | MassIVE
ProteomicsStructural dynamics in the evolution of SARS-CoV-2 spike glycoprotein: HDX-MS analysis of the spike of alpha, beta, delta and omicron variants - in apo state and in complex with the ACE2 receptor
The spike glycoprotein of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mediates binding to the ACE2 receptor and subsequent membrane fusion. It exists in a range of conformations, including a closed state unable to bind the ACE2 receptor, and an open state that does so but displa...
ORGANISM(S): Homo sapiens (Human) 
2023-03-15 | PXD039762 | Pride
Sars-cov-2 Spike glycoprotein Hdx-ms 
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