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MetabolomicsAspSnFR: A Genetically-Encoded Biosensor for Real-Time Monitoring of Aspartate in Live Cells

Aspartate is crucial for nucleotide synthesis, ammonia detoxification and maintaining redox balance via the malate-aspartate-shuttle (MAS). To disentangle these multiple roles of aspartate metabolism, tools are required that measure aspartate concentrations in real time and in live cells. We intr...

2024-08-27 | MTBLS10035 | MetaboLights
MetabolomicsOn-line targeted metabolomics for real-time monitoring of relevant compounds in fermentation processes
Fermentation monitoring is a powerful tool for bioprocess development and optimisation. On-line metabolomics is a technology that is starting to gain attention as a bioprocess monitoring tool, allowing the direct measurement of many compounds in the fermentation broth at a very high time resolution....
2024-03-05 | MTBLS7889 | MetaboLights
MetabolomicsStandardization procedures for real-time breath analysis by secondary electrospray ionization high-resolution mass spectrometry
Despite the attractiveness of breath analysis as a non-invasive means to retrieve relevant metabolic information, its introduction into routine clinical practice remains a challenge. Among all the different analytical techniques available to interrogate exhaled breath, secondary electrospray ionizat...
2020-01-11 | MTBLS842 | MetaboLights
MetabolomicsRapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites
Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that Secondary Electro-Spray Ionization-High Resolution Mass Spectrometry (SESI-HRMS) can be used as a diagnostic tool for rapid detection of bacterial ...
2022-07-06 | MTBLS5085 | MetaboLights
ProteomicsMoving Forward Real-Time Proteomics Mass Spectrometry by Remote Infrared Matrix-Assisted Laser Desorption-Ionization
Novel development makes remote real-time analysis with possible translation to in-vivo a reality. Remote Infrared Matrix Assisted Laser Desorption Ionization (Remote IR MALDI) system with endogenous water as matrix becomes real and allows to envisage real-time proteomics to be performed in the in-vi...
ORGANISM(S): Bos taurus (Bovine) Homo sapiens (Human) 
2018-04-20 | PXD008092 | Pride
Mass spectrometry • real-time analysis • remote ir maldi• proteins • charge state distribution 
ProteomicsSensitive neoantigen discovery by real-time mutanome-guided immunopeptidomics
Targeting cancer-specific HLA-peptide complexes is a promising immunotherapy approach, with mutated neoantigens offering high value for their immunogenicity and cancer-specificity. Selecting immunogenic targets requires personalized prioritization of cancer-specific immunopeptides. Mass spectrometry...
ORGANISM(S): Homo sapiens (Human) 
2025-06-20 | PXD059824 | Pride
Immunopeptidomics Real-time searches Antigen discovery 
ProteomicsThe GlycoPaSER prototype as a real-time N-glycopeptide identification tool based on the PaSER parallel computing platform
Real-time database searching allows for simpler and automated proteomics workflows as it eliminates technical bottlenecks in high throughput experiments. Most importantly, it enables results dependent acquisition (RDA) where search results can be used to guide data acquisition during acquisition. Th...
ORGANISM(S): Homo sapiens (Human) 
2023-03-13 | PXD040716 | Pride
Paser Glycoproteomics Glycopaser Results dependent acquisition (rda) Real-time search 
ProteomicsFull-featured, real-time database searching platform enables fast and accurate multiplexed quantitative proteomics.
Multiplexed quantitative analyses of complex proteomes enable deep biological insight. While a multitude of workflows have been developed for multiplexed analyses, the most quantitatively accurate method (SPS-MS3) suffers from long acquisition duty cycles. We built a new, real-time database search (...
ORGANISM(S): Homo sapiens (Human) 
2020-04-09 | PXD017823 | Pride
Multiplexed Orbiter Real-time search 
TranscriptomicsReal-time PCR analysis of microRNA expression in differentiating human skeletal muscle cells
Human skeletal myoblast cell line LHCN-M2 (Zhu et al., Aging Cell (2007), vol. 6, pp 515-523) were plated on collagen-coated 6-well Falcon cell culture plates at 500 000 cells/well, differentiation was induced 24h later by switching to DMEM supplemented with 0,01 mg/ml insulin and 0,1 mg/ml transfer...
ORGANISM(S): Homo sapiens 
2013-04-11 | E-GEOD-45957 | biostudies-arrayexpress
ProteomicsComparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm
The silkworm (Bombyx mori) has long been considered a source of food and medicine due to its high nutritional, medicinal, and economic value in East Asia. However, in some sensitive individuals, silkworm consumption can cause allergenic reactions such as vomiting, asthma, and anaphylaxis. Therefore,...
ORGANISM(S): Bombyx mori (Silk moth) 
2024-05-24 | PXD042494 | Pride
Real-time pcr Model cookies Alpha-amlyase Lipoprotein Bombyx mori Silkworm Lc-ms/ms 
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