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ProteomicsProteomics analysis of ligated and recombinant human histones H3 and H4
A library of unmodified and differentially modified human histones H3 and H4 was prepared using native chemical ligation as described previously (Bartke et al., 2010; Nakamura et al., 2019). The modification status of histone H3 and H4 products was confirmed by LC-MS/MS.
ORGANISM(S): Homo sapiens (Human) 
2023-12-10 | PXD020773 | Pride
Recombinant histones Ligated histones 
UnknownRapid purification of recombinant histones.
Not available
S-EPMC4121265 | biostudies-literature
GenomicsNovel nucleosomal particles containing core histones and linker DNA but no histone H1
The nucleosome plays a central role in genome regulation. Traditional methods for mapping nucleosomes depend on the resistance of the nucleosome core to micrococcal nuclease (MNase). However, the lengths of the protected DNA fragments are heterogeneous, limiting the accuracy of nucleosome position i...
ORGANISM(S): Synthetic plasmid 
2015-09-23 | E-GEOD-65889 | biostudies-arrayexpress
UnknownAntimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infections.
Not available
S-EPMC11575268 | biostudies-literature
UnknownA method for genetically installing site-specific acetylation in recombinant histones defines the effects of H3 K56 acetylation.
Not available
S-EPMC2856916 | biostudies-literature
UnknownSite-specific and regiospecific installation of methylarginine analogues into recombinant histones and insights into effector protein binding.
Not available
S-EPMC4260808 | biostudies-literature
ProteomicsALKBH7, a mitochondrial member of AlkB family, binds histones in vitro, part 1
Human genome encodes 9 AlkB family members, one of which is ALKBH7. In this study, we found recombinant ALKBH7 could bind bulk histones in GST pull-down and attenuate the signal of H4K20me2 in demethylation assays. With mass spectrometry, we also identified several potential ALKBH7-interacting prote...
ORGANISM(S): Homo sapiens (Human) 
2020-05-26 | PXD008318 | Pride
Alkbh7 mitochondrion histones 
UnknownTotal Chemical Synthesis of Modified Histones.
Not available
S-EPMC5810247 | biostudies-literature
UnknownPlasmodium falciparum histones induce endothelial proinflammatory response and barrier dysfunction.
Not available
S-EPMC3448071 | biostudies-literature
UnknownSelective Biological Responses of Phagocytes and Lungs to Purified Histones.
Not available
S-EPMC5413366 | biostudies-literature
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