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In E. coli, the pACYC-HA-Ub-E1 (UBA1)-UbcH5A-RNF126 and pET-22b-MIDN-His₆ plasmids were co-transformed. After IPTG induction to express all proteins required for ubiquitination, His-tagged MIDN was enriched with Ni-NTA resin and subjected to mass-spectrometric mapping of its ubiquitin-modified sites...
ORGANISM(S): Homo sapiens (Human) 
2026-06-01 | PXD069387 | Pride
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