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TranscriptomicsExpression profiling of the forming atrioventricular node using a novel Tbx3-based node-specific transgenic reporter
The atrioventricular (AV) node is a recurrent source of potentially life-threatening arrhythmias. Nevertheless, limited data are available on its developmental control or molecular phenotype. We used a novel AV node-specific reporter mouse to gain insight into the gene programs determining the forma...
ORGANISM(S): Mus musculus 
2009-06-02 | E-GEOD-13614 | biostudies-arrayexpress
TranscriptomicsA Transcriptomic Reporter Assay Employing Neutrophils to Measure Immunogenic Activity of Septic Patients' Plasma
This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
ORGANISM(S): Homo sapiens 
2014-03-17 | E-GEOD-49758 | biostudies-arrayexpress
TranscriptomicsSequential stage specific reporter line analysis (SSRLA) of 3 BAC transgenic mESC lines
FACS purified cells from differentiation day 14-15 cells from 3 BAC transgenic mESC lines: Hes::GFP (early), Nurr1::GFP (mid), and Pitx3::YFP (late) DA neuron development reporter lines All three lines were differentiated towards the midbrain dopamine phenotype, and FACS purification was performed ...
ORGANISM(S): Mus musculus 
2012-07-11 | E-GEOD-37446 | biostudies-arrayexpress
ProteomicsA Bioorthogonal Chemical Reporter for the Detection and Identification of Protein Lactylation
L-Lactylation is a recently discovered post-translational modification occurring on histone lysine residues to regulate gene expression. However, the substrate scope of lactylation, especially that in non-histone proteins, remains unknown, largely due to the limitations of current methods for analyz...
ORGANISM(S): Homo sapiens (Human) 
2022-06-23 | PXD033454 | Pride
Chemical reporter Chemical proteomics Lactylation 
GenomicsDNA-seq of an ELMSeq reporter cassette to study impact of C-terminal sequence variations on protein abundance
A ELMSeq reporter cassette was created to monitor Dam levels by methylation, and introduced in the genome. The regions of 6 nt upstream and 6 nt downstream the stop codon were randomized to study their effect on gene expression. The ELMSeq reporter cassette was composed of: promoter - dam - random N...
ORGANISM(S): Mycoplasma pneumoniae 
2020-05-04 | E-MTAB-8223 | biostudies-arrayexpress
GenomicsDNA-seq of ELMSeq reporter cassette
A ELMSeq reporter cassette was created to monitor Dam levels by methylation, and introduced in the genome. Some regions upstream dam were randomized to see their effect on gene expression. So the cassette consists in: 4xGATC sites - (promoter) - random region - dam. The amplicon was spanning the who...
ORGANISM(S): Mycoplasma pneumoniae 
2017-11-08 | E-MTAB-5365 | biostudies-arrayexpress
ProteomicsELM-seq - A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants
Quantitative analysis of the sequence determinants of transcription and translation regulation is of special relevance for systems and synthetic biology applications. Here, we developed a novel generic approach for the fast and efficient analysis of these determinants in vivo. ELM-seq (expression le...
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
2017-08-29 | PXD005606 | Pride
Screening Transcription Reporter Translation 
GenomicsAmplicon sequencing of splicing reporter mini-genes in a massively parallel reporter assay
Amplicon sequencing of splicing reporter mini-genes in a massively parallel reporter assay
PRJEB106948 | ENA
TranscriptomicsAmplicon sequencing of splicing reporter mini-genes in a massively parallel reporter assay
A library of cassette exon splicing reporter mini-genes was cloned under the control of five different promoters and tested, alongside pharmacological treatments affecting transcription, by transfection into HEK293 cells, isolation of the mRNA and targeted high-throughput sequencing of the reporter ...
ORGANISM(S): Homo sapiens 
2026-07-03 | E-MTAB-16553 | biostudies-arrayexpress
TranscriptomicsSystematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay
We apply a massively parallel reporter assay (MPRA) that relies on mRNA and plasmid tag sequencing (Tag-Seq) to compare the regulatory activities of more than 27,000 distinct variants of two inducible enhancers in human cells: a synthetic cAMP-regulated enhancer and the virus-inducible interferon be...
ORGANISM(S): Escherichia coli 
2012-02-26 | E-GEOD-31982 | biostudies-arrayexpress
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