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Retapamulin-assisted Ribo-seq reveals the alternative bacterial proteome
Unorthodox rules of extracting genetic information enable proteome expansion without increasing the genome size. The use of alternative translation initiation sites achieves this goal by allowing production of more than one protein from a single gene. Although several such examples have been serendi...
ORGANISM(S): Escherichia coli 
2019-03-04 | GSE122129 | GEO
Small proteome of Methanosarcina mazei: Insights from Ribo-seq and peptidomics under different nitrogen conditions
The ability to sequence genomes has far outstripped approaches for deciphering the information they encode. Here we present a suite of techniques, based on ribosome profiling (the deep-sequencing of ribosome-protected mRNA fragments), to provide genome-wide maps of protein synthesis as well as a p...
ORGANISM(S): Mus musculus 
The mesophilic methanogenic archaeal model organism Methanosarcina mazei strain Gö1, is crucial for climate and environmental research due to its ability to produce methane from H2 plus CO2, acetate, and methylamines. Here, we established the first Ribo-seq protocol for M. mazei strain Gö1 under two...
ORGANISM(S): Methanosarcina mazei Go1 
2024-08-02 | PXD045039 | Pride
To evaluate the expression of N-terminal proteoforms (protein isoforms with different N-terminus expressed from the same gene) in healthy human tissues, we re-analyzed public proteomics data of the draft map of the human proteome developed by the Pandey group (PRIDE project PXD000561). Using ionbot ...
ORGANISM(S): Homo sapiens (Human) 
2023-06-12 | PXD039339 | Pride
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