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GenomicsActive translatome profiling with RiboLace in MCF7 cells
Active translatome profiling with RiboLace in MCF7 cells
PRJNA445552 | ENA
GenomicsActive ribosome profiling with RiboLace and standard ribosome profiling in HEK-293 cells
Active ribosome profiling with RiboLace and standard ribosome profiling in HEK-293 cells
PRJNA445733 | ENA
TranscriptomicsActive translatome profiling with RiboLace in MCF7 cells
We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes and associate RNAs by means of an antibody-free and tag-free pull-down approach. RiboLace is fast, it works with very low input material and can be easily and rapidly used to obt...
ORGANISM(S): Homo sapiens 
2018-10-09 | GSE112295 | GEO
TranscriptomicsActive ribosome profiling from mouse brains with RiboLace
We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes by means of an antibody-free and tag-free pull-down approach. RiboLace is fast, it works with very low input material and can be easily and rapidly used to enhance Ribo-Seq, obta...
ORGANISM(S): Mus musculus 
2018-10-09 | GSE102354 | GEO
ProteomicsProteomics of MCF7 cells (LC-MS/MS Orbitrap Fusion Tribrid)
We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes and associate RNAs by means of an antibody-free and tag-free pull-down approach. We compared riboLace translation estimates with protein levels determined by proteomics in MCF7 c...
ORGANISM(S): Homo sapiens (Human) 
2018-11-21 | PXD009417 | Pride
Mcf7 Polysome profiling Pol-seq Translation Ribolace Rna-seq 
OtherActive ribosome profiling with RiboLace and standard ribosome profiling in HEK-293 cells
We developed RiboLace, a novel method based on a new puromycin-containing molecule, for the isolation of active ribosomes by means of an antibody-free and tag-free pull-down approach. RiboLace is fast, it works with very low input material and can be easily and rapidly used to enhance Ribo-Seq, obta...
ORGANISM(S): Homo sapiens 
2018-10-09 | GSE112353 | GEO
GenomicsMus musculus
Active ribosome profiling from mouse brains with RiboLace
PRJNA397543 | ENA
OtherRibosome profiling of SMN-primed ribosomes and active ribosome profiling from brains of a mouse model of severe SMA
Spinal muscular atrophy (SMA) is the most common genetic cause of infant mortality, with an incidence of around 1 in 6,000-10,000 live births. SMA is caused by low levels of full-length survival of motor neuron protein (SMN). We demonstrate that SMN binds to ribosomes and that this interaction is ti...
ORGANISM(S): Mus musculus 
2020-08-11 | GSE154106 | GEO
TranscriptomicsSingle-cell transcriptomics and translatomics dual-omics analysis reveals the potential mechanism of HFD-induced maternal RNA degradation abnormalities
This study explores the impact of high-fat diet (HFD)-induced obesity on maternal RNA degradation and its implications for oocyte quality and development in mice, using a novel transcriptome and translatome sequencing (T&T-seq) strategy. By combining RiboLace for translatome profiling and Smart-seq ...
ORGANISM(S): Mus musculus 
2025-04-14 | GSE294093 | GEO
GenomicsHomo sapiens
Comparison of the translatome of WT or eIF4E depleted HT1080 cells in normal and RSL3 treatment
PRJNA870904 | ENA
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