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ProteomicsLabel free quantitative mass spectrometry identifies platelet degranulation as an early event during platelet storage
Shelf life of platelet concentrates (PCs) is limited to up to 7 days due to loss of platelet functionality during storage, commonly referred to as the platelet storage lesion (PSL). To get more insight into the development of the PSL, we used label free quantitative mass spectrometry to identify cha...
ORGANISM(S): Homo sapiens (Human) 
2017-09-11 | PXD005610 | Pride
Proteomics Label free quantification Alpha-granules Platelet storage Platelets 
ProteomicsPU.1 interactome mouse bone marrow derived dendritic cells
Mouse bone marrow derived dendritic cells were generated by culturing bone marrow cells at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 5% FCS, 1% Pen/Strep, 5microM 2-mercaptoethanol, 20ng/ml GM-CSF. At day 7 dendritic cells were stimulated or not with 500 ng/ml LPS, and collected ...
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2018-11-30 | MSV000083176 | MassIVE
ProteomicsPU1 interactome in human monocyte derived dendritic cells
Human monocyte-derived DC were cultured at a density of 0.5x10E6 cells/ml in RPMI-1640 supplemented with 10% FCS, 1% Pen/Strep, 10 ng/ml GM-CSF and 10 ng/ml IL4. At day 7, they were stimulated or not with 500 ng/ml LPS. Cells were collected at day 10. Whole cell lystaes from 2x10E8 cells were used f...
ORGANISM(S): Homo Sapiens 
2018-11-30 | MSV000083175 | MassIVE
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