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GenomicsRNA polymerase II pausing downstream of core histone genes is different from genes producing polyadenylated transcripts
Recent genome-wide chromatin immunoprecipitation coupled high throughput sequencing (ChIP-seq) analyses performed in various eukaryotic organisms, analysed RNA Polymerase II (Pol II) pausing around the transcription start sites of genes. In this study we have further investigated genome-wide binding...
ORGANISM(S): Homo sapiens 
2012-06-15 | E-GEOD-34001 | biostudies-arrayexpress
GenomicsFunctional association of Gdown1 with RNA Polymerase II in Human
Most human genes are loaded with promoter proximally paused RNA polymerase II (RNAP II) molecules that are poised for release into productive elongation by P-TEFb. Gdown1, a protein that renders RNAP II responsive to mediator, is involved in RNAP II elongation control. During in vitro transcription ...
ORGANISM(S): Homo sapiens 
2012-01-13 | E-GEOD-32442 | biostudies-arrayexpress
GenomicsGenome-wide mapping of RNA Pol II binding site (GM12878)
We report the results of chromatin immunoprecipitation following by high-thoughput sequencing (ChIP-Seq) using the GA II platform from Illumina for the human RNA Polymerase II in GM12878 cells. We have also generated a sequenced input DNA datasets for GM12878 cells. For data usage terms and condit...
ORGANISM(S): Homo sapiens 
2009-12-31 | E-GEOD-19550 | biostudies-arrayexpress
GenomicsGlobal impact of RNA polymerase II elongation inhibition on alternative splicing regulation (ChIP-Seq)
The rate of RNA polymerase II (pol II) elongation can influence splice site selection in nascent transcripts, yet the extent and physiological relevance of this kinetic coupling between transcription and alternative splicing is not well understood. We performed experiments to perturb pol II elongati...
ORGANISM(S): Homo sapiens 
2010-12-09 | E-GEOD-25494 | biostudies-arrayexpress
TranscriptomicsProfiling of RNA Pol II occupancy in Drosophila neural stem cell populations using TaDa (Targeted DamID)
Cell-type specific transcriptional profiling is key to understanding cell fate specification and function. In order to achieve this it has been necessary, to date, to isolate specific cell types from complex tissues. We have developed 'TaDa', a technique that enables cell-specific profiling without ...
ORGANISM(S): Drosophila melanogaster 
2013-06-21 | E-GEOD-46803 | biostudies-arrayexpress
TranscriptomicsChIP-chip by array of yeast to determine genome-wide occupancy profiles for RNA polymerase (Pol) II, its phosphorylated forms, and transcription factors
To investigate the chromatin transcription cycle, we determined genome-wide occupancy profiles for RNA polymerase (Pol) II, its phosphorylated forms, and transcription factors in growing yeast. ChIP-chip was performed to identify the genomic binding locations for Rpb3, TFIIB, Tfg1, Kin28, Cet1, Spt4...
ORGANISM(S): Saccharomyces cerevisiae 
2010-08-23 | E-TABM-1033 | biostudies-arrayexpress
TranscriptomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (II)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20071 | biostudies-arrayexpress
GenomicsPol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (I)
Epigenetic control is an important aspect of gene regulation. Despite detailed understanding of many examples, the transcription of non-coding RNA genes by RNA polymerase (pol) III is less well characterized. Here we profile the epigenetic features of pol III target genes throughout the human gen...
ORGANISM(S): Homo sapiens 
2010-04-25 | E-GEOD-20040 | biostudies-arrayexpress
GenomicsHuman RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors.
RNA polymerase (Pol) III transcribes many noncoding RNAs (for example, transfer RNAs) important for translational capacity and other functions. We localized Pol III, alternative TFIIIB complexes (BRF1 or BRF2) and TFIIIC in HeLa cells to determine the Pol III transcriptome, define gene classes and r...
ORGANISM(S): Homo sapiens 
2010-04-14 | E-GEOD-20309 | biostudies-arrayexpress
TranscriptomicsPostrecruitment Regulation of RNA Pol II Directs Rapid Signaling Responses at the Promoters of Estrogen Target Genes
Under current models for signal-dependent transcription in eukaryotes, DNA-binding activator proteins regulate the recruitment of RNA polymerase II (Pol II) to a set of target promoters. Yet, recent studies, as well as our results herein, show that Pol II is widely distributed (i.e., "preloaded") a...
ORGANISM(S): Homo sapiens 
2010-05-14 | E-GEOD-13051 | biostudies-arrayexpress
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