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Alternative splicing significantly contributes to transcriptome complexity and has critical implications for cellular functions. Recent advancements in single-cell isolation and capture techniques have enabled high-throughput quantification of gene expression at single-cell resolution. Long-read seq...
ORGANISM(S): Mus musculus 
We performed direct cDNA sequencing in HeLa GFP∆Promoter cells by Oxford Nanopore Technology (ONT) on a MinION device to detect EGFP RNA levels after DSB induction.
ORGANISM(S): Homo sapiens 
Two sets of 10 samples from patients with CMML were used for this study. The first set (CMML11 to CMML20) includes 10 blood samples supplied by Eric Jourdan (Biological Resource Center CHU-Nimes, France). Peripheral blood mononuclear cells were separated by Ficoll-Hypaque density gradient and preser...
ORGANISM(S): Homo sapiens 
Here we describe CapTrap-Seq, an experimental workflow designed to address the problem of reduced transcript end detection by long-read RNA sequencing methods, especially at the 5' ends. We apply CapTrap-Seq to profile transcriptomes of the human heart and brain and we compared the obtained results ...
ORGANISM(S): Mus musculus 
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