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TranscriptomicsTranscription profiling of S. cerevisiae overexpressing transcriptional factors Kin28 and Pog1 suppresses the stress sensitivity caused by the rsp5 mutation
Rsp5 is an essential and multi-functional E3 ubiquitin ligase in Saccharomyces cerevisiae. We previously isolated the Ala401Glu rsp5 mutant, which is hypersensitive to various stresses. To understand the function of Rsp5 in stress responses, suppressor genes whose overexpression allows rsp5A401E cel...
ORGANISM(S): Saccharomyces cerevisiae 
2008-06-16 | E-GEOD-8729 | biostudies-arrayexpress
ProteomicsRsp5/Nedd4 is the major ubiquitin ligase that targets cytosolic misfolded proteins upon heat-stress
The heat-shock response is a complex cellular program that induces major changes in protein translation, folding and degradation to alleviate toxicity caused by protein misfolding. While heat-shock has been widely used to study proteostasis, it remained unclear how misfolded proteins are targeted fo...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2016-07-06 | PXD001214 | Pride
Rsp5 Heat-shock Cytosolic protein quality control 
ProteomicsInteractions between Yeast Proteins and Rsp5 Ubiquitin Ligase
To identify substrates of the ubiquitinating E3 enzyme Rsp5 we applied purified Rsp5 to duplicate protein arrays. The Rsp proteins were expressed as fusion proteins to GST. We used as a control Ubr1, a RING domain containing E3 ligase
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-30 | GSE14218 | GEO
ProteomicsInteractions between Yeast Proteins and Rsp5 Ubiquitin Ligase
To identify substrates of the ubiquitinating E3 enzyme Rsp5 we applied purified Rsp5 to duplicate protein arrays. The Rsp proteins were expressed as fusion proteins to GST. We used as a control Ubr1, a RING domain containing E3 ligase We analyzed Rsp5 from S.cerevisiae on duplicate arrays, with four...
ORGANISM(S): Saccharomyces cerevisiae 
2008-12-30 | E-GEOD-14218 | biostudies-arrayexpress
Proteomics1A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transporters
Cells rapidly remodel their proteomes to align their cellular metabolism to environmentalconditions. Ubiquitin E3 ligases enablethis response, by facilitatingrapid andreversible changes to protein stability, localization, or interaction partners. In S. cerevisiae, the GID E3 ligase ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-03-02 | PXD030902 | Pride
Gid Proteomics Nutrient signaling Dia Ms Ubiquitin e3 ligase Metabolism Rsp5 
GenomicsSaccharomyces cerevisiae
Interactions between Yeast Proteins and Rsp5 Ubiquitin Ligase
PRJNA111347 | ENA
UnknownUbp2 regulates Rsp5 ubiquitination activity in vivo and in vitro.
Not available
S-EPMC3777918 | biostudies-literature
ProteomicsRegulation of the Endocytosis and Prion Chaperoning Machineries by Yeast E3 Ubiquitin Ligase Rsp5 as Revealed by Orthogonal Ubiquitin Transfer
Attachment of the ubiquitin (UB) peptide to proteins via the E1-E2-E3 enzymatic machinery regulates diverse biological pathways, yet identification of the substrates of E3 UB ligases remains a challenge. We overcame this challenge by constructing an “orthogonal UB transfer (OUT) cascade with yeast E...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2022-10-13 | PXD023688 | Pride
Ubiquitin Lc-ms/ms 
TranscriptomicsOverexpression of transcriptional factors Kin28 and Pog1 suppresses the stress sensitivity caused by the rsp5 mutation
Rsp5 is an essential and multi-functional E3 ubiquitin ligase in Saccharomyces cerevisiae. We previously isolated the Ala401Glu rsp5 mutant, which is hypersensitive to various stresses. To understand the function of Rsp5 in stress responses, suppressor genes whose overexpression allows rsp5A401E cel...
ORGANISM(S): Saccharomyces cerevisiae 
2007-09-07 | GSE8729 | GEO
UnknownYeast ubiquitin ligase Rsp5 contains nuclear localization and export signals.
Not available
S-EPMC3167943 | biostudies-literature
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