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Glands were frozen at -80 ºC immediately after being retrieved and stored under this condition until initiating the proteomics analysis protocol. Glands were washed with cold PBS and immersed in 120 l of homogenization buffer (50 mM Tris-HCl, pH 6.8, 10 mM DTT, 4% (w/v) SDS). Glands were boiled for ...
ORGANISM(S): Trichonephila clavipes 
2022-12-07 | PXD023141 | Pride
Extracellular vesicles (EVs) are key players in intercellular communication. Here we show that modulating cellular environment by changing the available oxygen levels in vitro in embryonic-derived epicardial-derived cells (EPDCs) from mice we modulate their protein content. Murine EPDCs react by sec...
ORGANISM(S): Mus musculus (Mouse) 
2025-12-01 | PXD059112 | Pride
Health risks caused by stings from Vespa velutina nigrithorax (VV), also known as the yellow-legged Asian hornet, have become a public concern, but little is known about its venom composition. This study presents the proteome profile of the VV’s venom sac based on Sequential Window Acquisition of al...
ORGANISM(S): Vespa velutina nigrithorax 
2023-05-10 | PXD040764 | Pride
We performed a high-throughput, multiplexed quantitative proteomics analysis to determine protein abundance changes produced by BML-111 treatment in EAM and Ctrl mice. EAM was induced in 7 weeks-old BALB/c female mice by immunization at days 0 and 7 with a subcutaneous injection of 350 μg of murine ...
ORGANISM(S): Mus musculus (Mouse) 
2022-12-07 | PXD024345 | Pride
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