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TranscriptomicsMassive sequencing of hybrid DNA obtained by DNA:RNA immunoprecipitation (DRIP) in extracellular microvesicles (MV) produced by HeLa cells (using S9.6 antibody)
Hybrid (Bound) and non-hybrid (Unbound) DNA from Hela EV was isolated using DRIP procedure as follows. Between 1-5μg of nucleic acid DNA from EVs (DNase0 treated) was incubated overnight with 1μg of anti-DNA-RNA Hybrid (S9.6) antibody (KeraFast) under rotation at 4°C in 500μl of binding buffer (10mM...
ORGANISM(S): Homo sapiens 
2023-03-30 | E-MTAB-9634 | biostudies-arrayexpress
TranscriptomicsCUT&Tag for S9.6 in DIS3-depleted cells
CUT&Tag was performed to identify the effect of DIS3 depletion on DNA:RNA hybrids genome-wide. DIS3-AID HCT116 cells were treated with either Auxin (for rapid depletion of DIS3) or DMSO (control). CUT&Tag was performed targeting DNA:RNA hybrids using the CUT&Tag-IT® R-loop Assay Kit (Active Motive) ...
ORGANISM(S): Homo sapiens 
2026-08-03 | E-MTAB-17179 | biostudies-arrayexpress
Proteomicshuman HEK293T S9.6 Mass spectrometry
We are interested in proteins which could preferentially interact with DNA: RNA hybrids upon DNA damage. To achieve that, we treated HEK293T with 10 Gy IR, followed by 20 min recovery. We applied S9.6 antibody, which could recognize DNA: RNA hybrids structure, to isolate their interactome upon eithe...
ORGANISM(S): Homo sapiens (Human) 
2024-04-17 | PXD042271 | Pride
Dna: rna hybrids S9.6 Hek293t cells Human 
UnknownStructural basis of R-loop recognition by the S9.6 monoclonal antibody.
Not available
S-EPMC8960830 | biostudies-literature
GenomicsCUT&Tag for S9.6 in DIS3-depleted cells
CUT&Tag for S9.6 in DIS3-depleted cells
PRJEB115066 | ENA
UnknownS9.6 Antibody-Enzyme Conjugates for the Detection of DNA-RNA Hybrids.
Not available
S-EPMC10197132 | biostudies-literature
UnknownRecognition of RNA by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids.
Not available
S-EPMC8040515 | biostudies-literature
UnknownS9.6-based hybrid capture immunoassay for pathogen detection.
Not available
S-EPMC10728093 | biostudies-literature
OtherRecognition of cellular RNAs by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids
We showed that the majority of the signal observed in S9.6 immunofluorescence microscopy images of fixed human cells arises from RNA, not RNA:DNA hybrids. S9.6 staining is quantitatively unchanged by pre-treatment with the human RNA:DNA hybrid-specific nuclease, RNase H1, despite experimental verifi...
ORGANISM(S): Homo sapiens 
2021-03-17 | GSE141833 | GEO
GenomicsRecognition of cellular RNAs by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids
Recognition of cellular RNAs by the S9.6 antibody creates pervasive artifacts when imaging RNA:DNA hybrids
PRJNA594934 | ENA
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