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2D-LC/MS/MS analysis was used to examine time-dependent changes in proteome of E. coli O157:H7 strain Sakai upon abrupt downshifts from 35°C aw 0.993 to 14°C aw 0.967. Bacterial cells were harvested before abrupt downshifts in both temperature and aw (i.e. control), or at 0 (i.e. immediately after t...
ORGANISM(S): Escherichia coli 
2016-09-29 | PXD004803 | Pride
Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome ...
ORGANISM(S): Mus musculus 
2D-LC/MS/MS analysis was used to examine the proteome of E. coli O157:H7 strain Sakai during exponential growth under optimal condition (i.e., from 35°C aw 0.993). MS/MS data obtained from each protein sample were processed by the Computational Proteomics Analysis System (CPAS), a web-based system ...
ORGANISM(S): Escherichia coli 
2019-12-04 | PXD000583 | Pride
E. coli O157:H7 can use vegetables such as spinach as secondary hosts, from where they can be transmitted into the food chain. Our previous microarray analysis showed whole-scale changes in gene expression of E. coli driven in a large part in response to plant metabolites. The aim of this work was t...
ORGANISM(S): Escherichia coli 
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