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TranscriptomicsRNA-seq time-course of Calu-3 cells infected with SARS-CoV-2 early-lineage and Alpha variant viruses
Emergence of SARS-CoV-2 variants of concern (VOCs), including the globally successful Alpha (B.1.1.7) variant, suggests viral adaptation to enhance human-to-human transmission. Although much effort has focused on characterisation of spike changes, Alpha mutations outside spike likely contribute to a...
ORGANISM(S): Homo sapiens 
2021-12-31 | E-MTAB-11275 | biostudies-arrayexpress
Proteomicsiotin-streptavidin affinity purification mass spectrometry of proteins in human 293T cell using bioinylated Orf9b
COVID-19, caused by the new RNA virus strain SARS-CoV-2, is a serious acute respiratory disease. One of the prominent feature of SARS-COV-2 in terms of immune responses is that it triggers very low level responses of IFN-I in cells or COVID-19 patients. Previously SARS-CoV Orf9b were reported to be ...
ORGANISM(S): Homo sapiens (Human) 
2021-09-09 | PXD019803 | Pride
Ap-ms Human 293t cell Silac Sars-cov-2 orf9b 
ProteomicsSARS-CoV-2 variants evolve convergent strategies to remodel the host response: Affinity Purification Mass Spectrometry (APMS)
We sought to compare differences in the SARS-CoV-2 virus-human protein interaction networks between mutated (from the SARS-CoV-2 variants of concern) and wild-type viral proteins. Here, we ectopically expressed 2x-strep-tagged constructs encoding mutant or wild-type SARS-CoV-2 viral proteins in HEK2...
ORGANISM(S): Homo sapiens (Human) Severe acute respiratory syndrome coronavirus 2 
2024-02-16 | PXD036798 | Pride
Omics Apms Lung Sars-cov-2 Host response Host Infection Covid-19 Protein-protein interactions 
ProteomicsProteins interacting with SARS-CoV-2 ORF9b
Identification of the host factors trigger ORF9b degradation
ORGANISM(S): Homo Sapiens 
2024-02-23 | PXD050055 |
ProteomicsThe Orf9b protein of SARS-CoV-2 modulates mitochondrial protein biogenesis - Yeast Data Set
Viruses often induce a complex rewiring of cellular physiology to maximize their propagation. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) expresses high amounts of the viral antagonistic protein Orf9b. Increased amounts and mutated versions of Orf9b were found to correlate with ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-08-30 | PXD039850 | Pride
Orf9b Mitochondrial import 
ProteomicsAltered Subgenomic RNA Abundance in SARS-CoV-2 B.1.1.7 Infections
SARS-CoV-2 lineage B.1.1.7 viruses are more transmissible, may lead to greater clinical severity, and result in modest reductions in antibody neutralization. Subgenomic RNA(sgRNA) is produced by discontinuous transcription of the SARS-CoV-2 genome. Applying our tool(periscope) to ARTIC Network Nanop...
ORGANISM(S): Homo sapiens (Human) Severe acute respiratory syndrome coronavirus 2 
2021-11-26 | PXD029954 | Pride
Sars-cov-2 B.1.1.7 Coronavirus 
ProteomicsCoronavirus protein interaction mapping in bat and human cells identifies molecular and genetic switches for immune evasion and replication
Coronaviruses, including SARS-CoV-2, can cause severe disease in humans, whereas reservoir hosts like Rhinolophus bats appear to remain asymptomatic for reasons that are not well-recognized. To understand how host-specific protein-protein interactions (PPIs) shape infection outcomes, we generated co...
ORGANISM(S): Severe acute respiratory syndrome coronavirus 2 Homo sapiens (Human) Bat coronavirus RaTG13 Severe acute respiratory syndrome coronavirus 2 
2026-06-29 | PXD065873 | Pride
Host-pathogen interactions Ratg13 Sars-cov-2 Apms Bats 
TranscriptomicsGenotyping amplicons from SARS-CoV-2 viruses to verify the identity of PCR products
We used endpoint PCR to verify the presence of a new subgenomic RNA in SARS-CoV-2 (termed N.iORF3) and verify using nanopore sequencing that this is expressed via a newly evolved transcription regulatory sequence (TRS). We further show that this encodes a truncated C-terminal portion of Nucleocapsid...
ORGANISM(S): Severe acute respiratory syndrome-related coronavirus 
2025-01-01 | E-MTAB-14681 | biostudies-arrayexpress
ProteomicsBetacoronavirus Internal protein as a determinant of pathogenicity
Human betacoronaviruses encode an Internal (I) protein via an alternative reading frame within the nucleocapsid (N) gene, namely ORF8b for Middle-East Respiratory Syndrome Coronavirus (MERS-CoV) and ORF9b for Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) and SARS-CoV-2. Previous reports s...
ORGANISM(S): Homo sapiens (Human) 
2023-10-09 | PXD042023 | Pride
Human Sars-cov-2 Lc-ms/ms Timstof pro 
UnknownThe Orf9b protein of SARS-CoV-2 modulates mitochondrial protein biogenesis.
Not available
S-EPMC10491932 | biostudies-literature
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