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TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 from the beginning of the stationary phase compared to mid-log phase
Burkholderia cenocepacia J2315 was grown in LB broth to the beginning of stationary phase. The expression profile was compared to cultures harvested in mid-log phase.
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2754 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 at the beginning of the stationary phase compared to mid-log phase
Burkholderia cenocepacia J2315 was grown at 37 degrees centigrade in LB broth to the beginning of stationary phase (18 hours incubation time).
The expression profile was compared to cultures harvested in mid-log phase (7 hours incubation time).
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2790 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 grown under low or atmospheric oxygen concentration conditions
B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at OD 0.3 under full aeration and then transferred into a 50 ml centrifuge tube and placed upright into a CampyGen Compact (Oxoid, Basingstoke, UK) plastic pouch containing the gas generating paper sachet. The pouch was sealed immed...
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2787 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 grown to mid-stationary phase in basal salt medium with glucose or glycerol substrates
B. cenocepacia J2315 was grown to mid-stationary phase in basal salt medium with two different substrates: 20 mM glucose or 40 mM glycerol. Cells were harvested after 30 hours incubation at 37 degrees centigrade.
The expression profile was compared to cells grown on the same medium and harvested...
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2772 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 grown at 20C or 37C
B. cenocepacia J2315 was grown on LB medium to mid-stationary phase at O.D. 0.5 at 150 rpm in a shaking incubator at two different temperatures: 37 degrees and 20 degrees centigrade.
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2789 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia ambifaria during four growth conditions which modulated enacyloxin secretion
Gene expression was examined during four growth conditions which modulated antimicrobial secretion in B. ambifaria AMMD. A minimal salts medium (BSM; Hareland et al. 1975. J Bacteriol 121:272-85) with 0.05% yeast extract was used for all comparisons with the carbon source (4 g/L) and growth conditio...
ORGANISM(S): Burkholderia ambifaria 
2011-07-13 | E-MEXP-2819 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 grown to mid-log phase in different media
B. cenocepacia J2315 was grown to mid-log phase in different media: LB broth, iso-sensitest broth, basal salt medium with glucose
at pH 7 and pH 6, basal salt medium at pH 7 with a reduced iron content, and basal salt medium with glycerol
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2762 | biostudies-arrayexpress
TranscriptomicsTranscription profiling by array of Burkholderia cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species
B. cenocepacia J2315 was exposed to heat stress and to stress form reactive oxygen species.
To expose the cultures to heat stress, cells were grown at 37ºC to an O.D. of 0.4 to 0.45 and then transferred into a different shaking incubator at 42.5ºC, incubated for 1 hour at 150 rpm and harvested.<...
ORGANISM(S): Burkholderia cenocepacia 
2012-01-01 | E-MEXP-2791 | biostudies-arrayexpress
GenomicsExperimental evolution of Pseudomonas aeruginosa biofilms exposed to furanone C-30 with and without tobramycin
The aim of this study is to evaluate the evolutionary robustness of the quorum sensing inhibitor (QSI) furanone C-30 for the treatment of P. aeruginosa biofilm infections. We repeatedly exposed P. aeruginosa biofilms to furanone C-30 (with or without tobramycin) in the synthetic cystic fibrosis sput...
ORGANISM(S): Pseudomonas aeruginosa 
2021-03-05 | E-MTAB-10124 | biostudies-arrayexpress
GenomicsIn vitro evolution of Pseudomonas aeruginosa AA2 biofilms in the presence of cystic fibrosis lung microbiome members
P. aeruginosa was cultured in a MultiScreen-Mesh plate, which has a filter at the bottom of the wells. The plate was immersed in either in medium alone (control) or in medium inoculated with a mixture of five bacterial strains commonly found in cystic fibrosis sputum (\"microbiome\"). The filter pre...
ORGANISM(S): Pseudomonas aeruginosa 
2019-09-13 | E-MTAB-7331 | biostudies-arrayexpress
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