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An Affimetrix GeneChip Drosophila genome 2.0 array was used to study the effect of protocatechuic aldehyde on gene expression. The addition of 0.1 mM protocatechuic aldehyde to Drosophila S2 cells significantly affected the expression of 52 genes, with 29 being up-regulated and 23 being down-regulat...
ORGANISM(S): Drosophila melanogaster 
FLAG-VISTA expressed in Y-MESO-14 or NCI-H2373 cells was immunoprecipitated with anti-FLAG antibody. Parental cells were used for control samples. Immunoprecipitates were analyzed by LC-MS/MS.
ORGANISM(S): Homo Sapiens (human) 
Analysis of the peptides and proteins synthesized in a cell-free translation system containing either the natural tRNA extract, the hybrid-SL tRNA set, or the hybrid-SL tRNA set minus one chimeric tRNA using LC-MS.
ORGANISM(S): Cellular Organisms 
Alkaline phosphatase (ALP) is known to be a marker for several somatic stem cells and cancer cells. We found that human squamous cell carcinoma HeLa cells are comprised by ALP-positive and negative cells. Single cell-derived colony assay revealed that the former cells are labile with respect to ALP ...
ORGANISM(S): Homo sapiens 
Proteomic analysis was performed on desmoid tumors developed in Ctnnb1 or Ctnnb1/Smad4 compound mutant mice.
ORGANISM(S): Mus Musculus (mouse) 
Tda1 in Saccharomyces cerevisiae is a protein kinase that is activated in response to glucose starvation. However, how it is activated has not been investigated for a long time. In this analysis, yeast cells were grown in high (2%) or low (0.05%) glucose medium, and the lysate were subjected to immu...
ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast) 
Grainyhead-like transcription factor 3 (GRHL3) directs surface ectoderm differentiation under the control of the canonical Wnt/β-catenin pathway. However, the molecular mechanisms that control nuclear GRHL3 expression through β-catenin are not fully understood. Here, we show that the essential for m...
ORGANISM(S): Homo Sapiens (human) 
Plant (Arabidopsis thaliana) proteins that had been extracted by interaction using molecular probes and inhibitors derived from the 2,6-Dihalopurine
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
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