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TranscriptomicsSaccharomyces cerevisiae deletion collections chemogenomic screen with sr7575
Growth assay in the presence of a toxic chemical (sr7575) that uses the barcoded collections of yeast gene deletions (haploid, diploid, DamP) to identify deletion strains that are hypersensitive to the drug. Two-condition experiment – growth of a pool of strains in the presence or absence of the d...
ORGANISM(S): Saccharomyces cerevisiae 
2015-12-09 | E-GEOD-60934 | biostudies-arrayexpress
ProteomicsEvaluation of affinity purified RNA-protein complexes related with NMD
Nonsense-mediated mRNA decay (NMD) is a conserved RNA degradation pathway that is involved in development, resistance to viral infections and evolution. Upf1, a core NMD factor, is associated with a large variety of cellular RNA in complex ribonucleoprotein particles. We characterized NMD complexes ...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2023-08-30 | PXD028008 | Pride
Saccharomyces cerevisiae Rna Rnp Upf1 Yeast 
ProteomicsQuantitative proteomics of affinity purified yeast NMD complexes
Nonsense-mediated mRNA decay (NMD) is a major translation-dependent RNA degradation pathway required for embryo development and telomere maintenance. Core NMD factors Upf1, Upf2 and Upf3 are conserved from yeast to mammals but a model of the NMD machinery compatible with all eukaryotes is not yet av...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2018-03-12 | PXD007159 | Pride
Ltq-orbitrap velos Lc-ms/ms Affinity purification Yeast Maxquant Label-free Tap 
TranscriptomicsQuality control of transcription start site selection by Nonsense-Mediated-mRNA Decay
Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA quality-control pathway targeting transcripts such as messenger RNAs harboring premature stop-codons or short upstream open reading frame (uORFs). Our transcription start sites (TSSs) analysis of Saccharomyces cerevisiae cells deficie...
ORGANISM(S): Saccharomyces cerevisiae BY4741 
2015-04-24 | E-GEOD-64139 | biostudies-arrayexpress
TranscriptomicsGenetic interaction mapping (GIM) for factors involved in RNA and protein synthesis and degradation
This is a partial data set that corresponds to 16 GIM screen results. A larger data set, using a different microarray platform is included in a separate submission. The results correspond to relative growth defect estimates for combinations of a given mutant with more than 5000 other yeast (Saccharo...
ORGANISM(S): Saccharomyces cerevisiae S288C 
2019-06-30 | E-MTAB-7191 | biostudies-arrayexpress
TranscriptomicsExpression analysis of DamP mRNAs using expressed molecular barcodes
We have generated 979 yeast strains in which the natural 3' UTR of essential gene mRNAs has been replaced by the same long 1.4 kb artificial 3' UTR (DAmP modification). Nonsense mediated mRNA decay (NMD) of these mRNA reporters was tested by using Agilent barcode microarrays by taking advantage of m...
ORGANISM(S): Saccharomyces cerevisiae 
2014-01-10 | E-GEOD-53954 | biostudies-arrayexpress
UnknownNonsense-mediated mRNA Decay Involves Two Distinct Upf1-bound Complexes
Not available
S-SCDT-EMBOJ-2018-99278 | biostudies-other
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