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ProteomicsTime-resolved proteomic profiling for CHO-K1 fed-batch cultivations
The majority of recombinant protein therapeutics are produced with Chinese Hamster Ovary (CHO) cells. Productivity depends on the initial cell line engineering in terms of integration site or choice of an appropriate promotor for the recombinant gene expression, as well as media and process paramete...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-01-25 | PXD018439 | Pride
Fedbatch cultivation Nlc-ms/ms Cho Proteomics Dda 
ProteomicsPhospho and expression proteomics in chinese hamster ovary cells
We investigated CHO signaling events in a comparative expression and phospho proteomics approach of recombinant mAb producing XL99 cell line and according parental cells. First, on proteomic level differences between producer and parental cells were evaluated by label-free quantification of proteins...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2020-02-26 | PXD010537 | Pride
Phosphoproteomics Cho Silac Lfq Proteomics Lc-msms 
ProteomicsHyperosmolality in CHO Culture: Effects on Proteome
Chinese hamster ovary cells (CHO) were exposed to highly concentrated feed solution during a fed-batch cultivation which causes an unphysiological osmolality increase (>300 mOsm/kg) affecting cell physiology, morphology and proteome. To get a deeper insight into underlying molecular mechanisms invol...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2022-05-20 | PXD027443 | Pride
Nlc-ms lfq proteomics Fed-batch Q exactive plus Cho Hyperosmolality Cell size 
ProteomicsTECAN Resolvex A200 Positive Pressure Workstation for Semi Automated Peptide Purification of Complex Proteomic Samples
High-throughput has become a substantial need in many omics investigations. In proteomics the sample preparation workflow consists of multiple steps, whereby with each additional manual step more bias is added to the sample. Especially for label-free quantification experiments this drastically reduc...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-01-25 | PXD018444 | Pride
Peptide purification Ppspe Cell culture Fedbatch cultivation High-throughput proteomics Sample preparation 
ProteomicsIsolation and Characterization of small EVs from CHO-K1 cell cultures
Chinese hamster ovary (CHO) cells are widely used host cells for recombinant protein production and currently the most commonly utilized mammalian organism in large scale biopharmaceutical production. Since the discovery of exosomes as a new type of small extracellular vesicles (EVs) by Johnstone et...
ORGANISM(S): Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) 
2021-09-09 | PXD018446 | Pride
Proteomics Cho Cell culture Extracellular vesicles Exosomes 
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