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Preparation of nuclear extracts

D. melanogaster Schneider cells were prepared as previously described by Andrews and Faller (1991). Briefly, in a typical preparation, 8x106 cells (grown in Schneider medium plus 10% fetal calf serum; GIBCO-BRL) were pelleted and resuspended in 1,5 mL cold PBS1...
ORGANISM(S): Drosophila melanogaster 
Forum domains are stretches of chromosomal DNA that are excised from eukaryotic chromosomes during their spontaneous non-random fragmentation. Mostly forum domains are of 50-200 kb in length, although larger domains, up to 500 - 700 kb, are also observed.

We performed a genome-wide mapping o...
ORGANISM(S): Drosophila melanogaster 
Wilms tumour (nephroblastoma) is the most common paediatric renal tumour, affecting 1 in 10,000 children. Despite the excellent survival of patients treated with a combination of surgery, chemotherapy and radiotherapy, approximately 15% will relapse and die of this disease. We have identified CACNA1...
ORGANISM(S): Homo sapiens 
In order to identify interaction partner of the Drosophila melanogaster TFIIA protein, we have immunoprecipitated an endogenously 3xFLAG-AID tagged TFIIA-L from Drosophila Schneider S2 cells
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2023-02-16 | PXD028094 | Pride
Profiling of changes in steady state RNA levels upon RNAi-mediated knockdown of the nucleosome remodeling ATPase ISWI in Drosophila SL2 cells. Quantitation of the corresponding proteomes has been performed to describe transcriptome-proteome relations. The analysis indicates a limited correlation of ...
ORGANISM(S): Drosophila melanogaster 
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