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To analyze the impact of elevated c-di-GMP concentrations in P. aeruginosa, we expressed pleD* on an inducible vector (pHERD20T) in the PAO1 wild-type strain. PleD is a DGC from Caulobacter cresentusand the pleD* construct variant encodes for a constitutively active enzyme due to four amino acid exc...
ORGANISM(S): Pseudomonas aeruginosa PAO1 
2022-07-25 | PXD027098 | Pride
Nutrient availability, in particular that of carbon (C) and nitrogen (N), is one of the most important factors for the regulation of plant metabolism and development. In addition to independent utilization, the ratio of C to N metabolites in the cell is also important for the regulation of plant gro...
ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress) 
Tissue powder was separated on a non-aqueous gradient to fractionate proteins and metabolites for subcellular locations. In total, twelve fractions (01-12) were collected from three independent gradients (A, B, C)
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2018-10-24 | PXD000609 | Pride
Quorum sensing (QS) is a mechanism of intercellular communication that enables microbes to alter gene expression and adapt to the environment. While QS has been extensively studied in bacteria, little is known about cell-cell communication in archaea. We could show that for Haloferax volcanii, the t...
ORGANISM(S): Haloferax volcanii (Halobacterium volcanii) Archaea 
2025-06-17 | PXD059278 | Pride
Nitrate nutrtition was withdrawn from Arabiodpsis plants to study early responses upon nitrate depletion. Plants were grown under full nutrition (3mM nitrate) for three weeks and then transferred to nutrient solution without nitrate for 15 minutes and 3 hours.
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-21 | PXD015390 | Pride
Cell shape plays a crucial role in microbial survival. While Haloferax volcanii, a model haloarchaeon, forms rods and disks, depending on environmental conditions, little is known about mechanisms underpinning archaeal cell-shape determination. We identified mutants that exclusively form rods and ca...
ORGANISM(S): Haloferax volcanii (Halobacterium volcanii) Archaea 
2024-02-17 | PXD040781 | Pride
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