Sort   by:  
 Page size 
ProteomicsAutomated SDS depletion for mass spectrometry of membrane proteins through transmembrane electrophoresis
Membrane proteins are underrepresented in proteome analysis platforms because of their hydrophobic character, contributing to decreased solubility. Sodium dodecyl sulfate is a favored denaturant in proteomic workflows, facilitating cell lysis and protein dissolution. However, SDS impedes MS detect...
ORGANISM(S): Escherichia coli 
2016-07-07 | PXD003941 | Pride
Ltq E.coli Membrane proteins 
ProteomicsBovine Liver Membrane Preparation
We optimized an SDS depletion protocol involving KCl to precipitate the surfactant from intact proteins in the presence of urea at high pH. Provided is a comparison of 4 samples preciipitated at low pH (8) vs high pH (12), with (8 M) vs without (0 M) urea.
ORGANISM(S): Bos taurus (Bovine) 
2025-08-04 | PXD061512 | Pride
Urea Ph Sds depleted Kcl preciptation 
UnknownMulti-Step Unfolding and Rearrangement of α-Lactalbumin by SDS Revealed by Stopped-Flow SAXS.
Not available
S-EPMC7366515 | biostudies-literature
ProteomicsComparison of the MCF7 proteome cultured in standard FBS and EV depleted FBS
COmparison of the MCF7 proteomes after being cultured for 3 d in either 10% FBS (standard, commercial) or in 10% EV-depleted FBS (EDS, commercial) to validate the absence of EV specific proteins in EDS to exclude potential cross-contamination of xeno-EVs
ORGANISM(S): Bos taurus (Bovine) 
2025-05-06 | PXD051956 | Pride
Ev-depleted fbs Extracellular vesicles Starvation Fbs 
ProteomicsAnticancer effect of deuterium depleted water - comprehensive proteomics analysis suggests a redox disbalance mechanism
The proteomics data is derived from 4 parts of samples prepared and analysed by nanoLC-MS/MS. In order to make appropriate statistical analysed could be performed, all treatments were performed in biological replicates. The number of replicates was 4 for protein abundances analysis (part 1), 2 for t...
ORGANISM(S): Homo sapiens (Human) 
2019-09-26 | PXD011085 | Pride
Anticancer Proteomics Redox disbalance Deuterium depleted water 
ProteomicsExercise training decreases the load and changes the content of circulating SDS-resistant protein aggregates in patients with heart failure with reduced ejection fraction
Heart failure (HF) is often associated with the deterioration of the protein quality control (PQC) system and the accumulation of protein aggregates. Evidence from tissue biopsies showed that exercise restores PQC system in HF; however, little is known about its effects on plasma proteostasis. Our s...
ORGANISM(S): Homo sapiens (Human) 
2025-05-06 | PXD045161 | Pride
Hfref  exercise training  protein aggregates  proteomics 
UnknownThe ubiquitin-like protein HUB1 forms SDS-resistant complexes with cellular proteins in the absence of ATP.
Not available
S-EPMC1326417 | biostudies-literature
ProteomicsEvidence of immune modulators in the secretome of the equine tapeworm Anoplocephala perfoliata
Anoplocephala perfoliata is a neglected gastro-intestinal tapeworm, commonly infecting horses worldwide. Molecular investigation of A. perfoliata is hampered by a lack of tools to better un-derstand the host-parasite interface. This interface is likely influenced by parasite derived immune modulator...
ORGANISM(S): Anoplocephala perfoliata 
2021-07-26 | PXD027105 | Pride
Anoplocephala perfoliata  secretome  extracellular vesicles  ev surface  ev depleted esp  lcmsms 
UnknownSds22 regulates aurora B activity and microtubule–kinetochore interactions at mitosis
Not available
S-JCBD-200912046 | bioimages
TranscriptomicsMeiosis timecourse Rap1-depletion Rap1 ChIP-chip
To confirm that Rap1-depletion resulted in decreased binding of Rap1 to its target sites, we performed compared Rap1 ChIP-chip experiments in respiratory cells (pre-meiotic/YPA 20 hours) depleted (plus doxycycline for 12 hours) or not depleted (minus doxycycline) of Rap1. There is growing recognitio...
ORGANISM(S): Saccharomyces cerevisiae SK1 
2016-09-01 | E-GEOD-75260 | biostudies-arrayexpress
Sort   by:  
 Page size