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Sodium dodecyl sulfate (SDS) is widely used in sample preparation for proteomic research. However, SDS is incompatible with liquid chromatography and electrospray ionization (ESI). SDS depletion is therefore required ahead of liquid chromatography coupled with mass spectrometry (LC-MS) analysis. Mos...
ORGANISM(S): Homo Sapiens Mus Musculus 
2021-03-02 | PXD023557 |
Membrane proteins are underrepresented in proteome analysis platforms because of their hydrophobic character, contributing to decreased solubility. Sodium dodecyl sulfate is a favored denaturant in proteomic workflows, facilitating cell lysis and protein dissolution. However, SDS impedes MS detect...
ORGANISM(S): Escherichia coli 
2016-07-07 | PXD003941 | Pride
Chickpea (Cicer arietinum L.) seed proteins show a lot of functional properties leading this leg-ume an interesting component for the development of protein-enriched foods. However, both in-depth proteomic investigation and structural characterization of chickpea proteins seed are still lacking. In ...
ORGANISM(S): Cicer arietinum (Chickpea) (Garbanzo) 
2024-06-23 | PXD049382 | Pride
Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample p...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2021-02-26 | PXD022688 | Pride
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