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GenomicsSuper Elongation Complex (SEC) and global genomic analyses in murine embryonic stem (ES) cells and in human cells in response to activation signals.
Transcriptional regulation of developmentally controlled genes is at the heart of differentiation and organogenesis. In this study, we have performed global genomic analyses in murine embryonic stem (ES) cells and in human cells in response to activation signals. We have identified an essential role...
ORGANISM(S): Mus musculus 
2011-07-19 | E-GEOD-30267 | biostudies-arrayexpress
TranscriptomicsGene target specificity of the Super Elongation Complex (SEC) family: How HIV-1 Tat employs selected SEC members to activate viral transcription
The AF4/FMR2 proteins AFF1 and AFF4 act as a scaffold to assemble the Super Elongation Complex (SEC) that strongly activates transcriptional elongation of HIV-1 and cellular genes. Although they can dimerize, it is unclear whether the dimers exist and function within a SEC in vivo. Furthermore, it i...
ORGANISM(S): Homo sapiens 
2015-05-20 | E-GEOD-69021 | biostudies-arrayexpress
ProteomicsQuantitative proteomic comparison between wild type Col and sec-5 mutant using 15N metabolic labeling
SECRET AGENT (SEC) is an O-GlcNAc transferase involved in O-GlcNAcylation of many proteins. However, sec-5 null allele mutant phenotype is very subtle. To examine if there are proteins that show altered expression in the sec-5 mutant compared to wild-type Col, we designed MS experiments to identify ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-02-04 | PXD030575 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Secret agent (sec) 
TranscriptomicsThe SEC family of RNA Polymerase II elongation factors: gene target specificity and transcriptional output
The elongation stage of transcription is a highly regulated in metazoan. We previously purified the AFF1/AFF4-containing Super Elongation Complex (SEC) as a major regulator of development and cancer pathogenesis. Here, we report the biochemical isolation of SEC-like 2 (SEC-L2) and SEC-like 3 (SEC-L3...
ORGANISM(S): Homo sapiens 
2012-05-01 | E-GEOD-34097 | biostudies-arrayexpress
ProteomicsE. coli expressed SECRET AGENT O-GlcNAc modifies threonine 829 of GIGANTEA
The Arabidopsis thaliana glycosyl transferases SPINDLY (SPY) and SECRET AGENT (SEC) modify nuclear and cytosolic proteins with O-linked fucose or O-linked Nacetylglucosamine (O-GlcNAc), respectively. O-fucose and O-GlcNAc modifications can occur at the same sites. SPY interacts physically and geneti...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-07-15 | PXD053909 | Pride
Arabidopsis thaliana Gigantea Protein modification O-glcnac E. coli 
ProteomicsPost-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.
O-linked-β-N-Acetylglucosamine (O-GlcNAc) and O-fucose are two sugar-based post-translational modifications (PTMs) whose mechanistic role in plant signalling and transcriptional regulation is still largely unknown. Here, we investigated how two O-glycosyltransferase enzymes of Arabidopsis thaliana, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-22 | PXD037917 | Pride
Spy Spt O-glycosylation Gynoecium Sec 
ProteomicsPost-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.
O-linked-β-N-Acetylglucosamine (O-GlcNAc) and O-fucose are two sugar-based post-translational modifications (PTMs) whose mechanistic role in plant signalling and transcriptional regulation is still largely unknown. Here, we investigated how two O-glycosyltransferase enzymes of Arabidopsis thaliana, ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-01-22 | PXD043987 | Pride
Spy Mass spectrometry Spt O-glycosylation Gynoecium Sec 
ProteomicsQuantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F1 on Eclipse (EThcD)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discove...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-22 | PXD044832 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsQuantitative O-GlcNAc analysis between wild type and sec-5 using SILIA (F2 R1 on QE-HF)
O-GlcNAcylation is a crucial post-translational modification of proteins observed in both plants and animals and plays a key role in growth and development. Plants have close OGT homologs, SECRET AGENT (SEC) and SPINDLY (SPY). In vitro, SEC has shown O-GlcNAc activity. Recently, a surprising discove...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2024-05-22 | PXD044831 | Pride
Mass spectrometry O-glcnacylation Proteomics. Lectin weak affinity chromatography (lwac) 
ProteomicsPost-translational modification of the transcription factor SPATULA by SECRET AGENT and SPINDLY promotes Arabidopsis style development.
O-linked-β-N-Acetylglucosamine (O-GlcNAc) and O-fucose are two sugar-based post-translational modifications (PTMs) whose mechanistic role in plant signalling and transcriptional regulation is still largely unknown. Here, we investigated how two O-glycosyltransferase enzymes of Arabidopsis thaliana, ...
ORGANISM(S): Nicotiana tabacum (Common tobacco) 
2024-01-22 | PXD043957 | Pride
Spy Mass spectrometry Spt O-glycosylation Gynoecium Sec 
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