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ProteomicsLC/MS Analysis of Myc-Affinity Captured, on-Beads Crosslinked, and on-Beads Digested S. cerevisiae SCC2 subunit of cohesin loading factor
Myc-Affinity Capture and on-Beads Crosslinking For each Saccharomyces cerevisiae strain (C-terminally myc-tagged Scc2 subunit of the cohesin loading factor complex or wild-type BY4741), 2x 3 L YPD medium (add 300 ml 20% Glucose to each 3 L of YPD before using) were separately inoculated in a 5L flas...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2020-10-20 | MSV000086339 | MassIVE
ProteomicsMudPIT analyses of the proteins co-purified with Ada2b-PB-FLAG-HA FLAG-affinity purified from Drosophila melanogaster S2 cells
Protein Complexes Purification- Stable S2 cell lines expressing Ada2b (isoform B; NP_001027151.1) in the pRmHa3-CHA2FL2 (Ada2b-PBH2F2) were maintained at 1-2 e6 cells/mL in SFX medium (HyQ SFX-Insect). Cells stably transfected with empty vector served as negative controls. Protein complexes containi...
ORGANISM(S): Drosophila Melanogaster (ncbitaxon:7227) 
2018-11-20 | MSV000083153 | MassIVE
ProteomicsMudPIT LiPMS analyses of whole cell protein extracts from Saccharomyces cerevisiae grown at 21C and 35C
Sample Preparation. Three biological replicates of wild type yeast cells (BY4741) were cultured with YEPD either at 21C or 35C overnight (ca. 18hrs) to OD600 around 2. Pre-warmed/chilled fresh YEPD was next used to dilute the cultures, which were grown for an additional 6hrs to OD600 around 1. 120m...
ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932) 
2021-02-01 | MSV000086790 | MassIVE
TranscriptomicsControlling for gene expression changes in transcription factor protein networks.
The development of affinity purification technologies together with mass spectrometric analyses of the purified protein mixtures (AP-MS) has been used both to identify new protein-protein interactions and to define the subunit composition of protein complexes. Transcription factor protein interactio...
ORGANISM(S): Homo sapiens 
2014-04-24 | E-GEOD-57027 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of yeast treated with rapamycin in a 7-part time series
This experiment was a 7-part time series (or, 2x7 factorial design) examining the effect of rapamycin on gene expression in Saccharomyces cerevisiae. Treated and untreated cultures were compared at 0, 20, 40, 60, 120, 240, and 360 minutes. In the paper, expression ratios (mRNA) at each time point ...
ORGANISM(S): Saccharomyces cerevisiae 
2010-02-05 | E-TABM-639 | biostudies-arrayexpress
TranscriptomicsExamination of gene expression in human breast cancer cells exposed to the HDAC inhibitor SAHA
The identification of proteins that change in response to a drug perturbation can shed light on the molecular mechanisms of the drug and its potential use in therapies. Histone deacetylases (HDACs) are targets for cancer therapy. Suberoylanilide hydroxamic acid (SAHA) is an FDA approved HDAC inhibit...
ORGANISM(S): Homo sapiens 
2014-09-01 | E-GEOD-60124 | biostudies-arrayexpress
TranscriptomicsExamination of gene expression in response to ING2 knockdown by siRNA in human breast cancer cells
The Sin3/HDAC multi-protein complex consists of at least 17 subunits and is known to have roles in diverse biological and cellular processes including transcription, chromatin structure, and the cell cycle. ING2 is a non-catalytic component of this complex. To obtain a better mechanistic understandi...
ORGANISM(S): Homo sapiens 
2014-09-01 | E-GEOD-60123 | biostudies-arrayexpress
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