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MetabolomicsComprehensive High-Resolution Multiple-Reaction Monitoring Mass Spectrometry for Targeted Eicosanoid Assays
Eicosanoids comprise a class of bioactive lipids derived from a unique group of essential fatty acids that mediate a variety of important physiological functions. Owing to the structural diversity of these lipids, their analysis in biological samples is often a major challenge. Advancements in mass ...
2018-06-26 | MTBLS641 | MetaboLights
MetabolomicsLiquid Chromatography-Mass Spectrometry Quantification of Phytochemicals in Withania somnifera Using Data-Dependent Acquisition, Multiple-Reaction-Monitoring, and Parallel-Reaction-Monitoring with an Inclusion List

Characterization of botanical extracts by mass spectrometry-based metabolomics analysis helps in determining the phytochemical composition that underlies their bioactivity and potential health benefits, while also supporting reproducibility of effects in clinical trials. The quantification of sev...

2024-07-10 | MTBLS10418 | MetaboLights
MetabolomicsMultiomics and digital monitoring during lifestyle changes reveal independent dimensions of human biology and health

In order to explore opportunities for personalized and predictive health care, we collected serial clinical measurements, health surveys and multiomics profiles (genomics, proteomics, autoantibodies, metabolomics and gut microbiome) from 96 individuals. The participants underwent data-driven heal...

2021-09-02 | MTBLS2187 | MetaboLights
MetabolomicsAspSnFR: A Genetically-Encoded Biosensor for Real-Time Monitoring of Aspartate in Live Cells

Aspartate is crucial for nucleotide synthesis, ammonia detoxification and maintaining redox balance via the malate-aspartate-shuttle (MAS). To disentangle these multiple roles of aspartate metabolism, tools are required that measure aspartate concentrations in real time and in live cells. We intr...

2024-08-27 | MTBLS10035 | MetaboLights
MetabolomicsWT 2.0: Unveiling the “dark matter” in the metabolome using all-ion stepwise fragmentation acquisition mode (ASFAM) and deep learning-based feature extraction

Current metabolomics methods often miss low-abundance compounds and yield incomplete or ambiguous MS2 spectra, resulting in the presence of “dark matter” within the metabolome. Here, we introduce WT 2.0, which employs an all-ion stepwise fragmentation acquisition mode (ASFAM) to acquire comprehe...

2025-09-23 | MTBLS13023 | MetaboLights
ProteomicsProtein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS) C4PR_LIV
Protein turnover represents an important mechanism in the functioning of cells, with deregulated synthesis and degradation of proteins implicated in many diseased states. Therefore, proteomics strategies to measure, with high confidence, turnover rates are of vital importance to understanding many ...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-10 | PXD004413 | Pride
Srm Proteomics Ms1 Mass spectrometry Protein turnover Quantification 
ProteomicsDirect and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring HUB_MAN C4PR_LIV
Defining intracellular protein concentration is critical in molecular systems biology. Although strategies for determining relative protein changes are available, defining robust absolute values in copies per cell has proven significantly more challenging. Here we present a reference dataset quantif...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-12-11 | PXD002694 | Pride
Absolute quantification  selected reaction monitoring  srm  stable-isotope labelling  yeast  tandem quadrupole  qconcat 
ProteomicsMultiplex selected reaction monitoring assay for quantification of 15 human tissue kallikreins in biological samples
Human tissue kallikreins (KLKs) are a group of 15 secreted serine proteases encoded by the largest contiguous cluster of protease genes in the human genome. KLKs are involved in coordination of numerous physiological functions including regulation of blood pressure, neuronal plasticity, skin desquam...
ORGANISM(S): Homo sapiens (Human) 
2016-07-08 | PXD003324 | Pride
Cervicovaginal fluid Sweat Klk4 secretion Mass spectrometry Seminal plasma Selected reaction monitoring Kallikreins 
MetabolomicsGNPS - Data files for manuscript - Selected ion monitoring for orbitrap-based metabolomics
Please open README-data-for-SIM-paper-20240210.doc for details.
ORGANISM(S): Mus Musculus (ncbitaxon:10090) 
2024-02-14 | MSV000094084 | GNPS
ProteomicsAbsolute protein quantification of Mycoplasma pneumoniae proteome by combining selected reaction monitoring of a reduced set of labeled peptides and label-free shotgun mass spectrometry.
We quantified the protein abundances of Mycoplasma pneumoniae (Mpn), a genome-reduced organism, by combining selected reaction monitoring of a reduced set of labeled peptides and label-free shotgun mass spectrometry. A set of 77 labeled peptides were synthesized and used as internal standard peptide...
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
2023-08-31 | PXD035159 | Pride
Mycoplasma pneumoniae Absolute quantification Label-free Selected reaction monitoring 
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