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MetabolomicsLiquid Chromatography-Mass Spectrometry Quantification of Phytochemicals in Withania somnifera Using Data-Dependent Acquisition, Multiple-Reaction-Monitoring, and Parallel-Reaction-Monitoring with an Inclusion List

Characterization of botanical extracts by mass spectrometry-based metabolomics analysis helps in determining the phytochemical composition that underlies their bioactivity and potential health benefits, while also supporting reproducibility of effects in clinical trials. The quantification of sev...

2024-07-10 | MTBLS10418 | MetaboLights
MetabolomicsComprehensive High-Resolution Multiple-Reaction Monitoring Mass Spectrometry for Targeted Eicosanoid Assays
Eicosanoids comprise a class of bioactive lipids derived from a unique group of essential fatty acids that mediate a variety of important physiological functions. Owing to the structural diversity of these lipids, their analysis in biological samples is often a major challenge. Advancements in mass ...
2018-06-26 | MTBLS641 | MetaboLights
ProteomicsA Selected Reaction Monitoring Approach for Validating Candidate Biomarkers
We here describe a Selected Reaction Monitoring (SRM)-based approach for the discovery and validation of peptide biomarkers for cancer. The first stage of this approach is the direct identification of candidate peptides through comparison of proteolytic peptides derived from the plasma of cancer p...
ORGANISM(S): Homo sapiens (Human) 
2017-12-11 | PXD008094 | Pride
Diagnostics Clinical proteomics Targeted detecton Quantitative proteomics 
ProteomicsLarge-Scale Targeted Proteomics Using Internal Standard Triggered-Parallel Reaction Monitoring
Development, implementation, and evaluation of a new data acquisition scheme called internal standard triggered-parallel reaction monitoring (IS-PRM) to increase the scale of targeted quantitative experiments while retaining high detection and quantification performance. All the details about the da...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2017-03-31 | MSV000080832 | MassIVE
ProteomicsDirect and Absolute Quantification of over 1800 Yeast Proteins via Selected Reaction Monitoring HUB_MAN C4PR_LIV
Defining intracellular protein concentration is critical in molecular systems biology. Although strategies for determining relative protein changes are available, defining robust absolute values in copies per cell has proven significantly more challenging. Here we present a reference dataset quantif...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2015-12-11 | PXD002694 | Pride
Absolute quantification  selected reaction monitoring  srm  stable-isotope labelling  yeast  tandem quadrupole  qconcat 
ProteomicsTargeted Quantification of Proteoforms in Human Samples by Proteoform Reaction Monitoring
Here, we describe an approach based on the principle of PRM for the measurement of intact proteoforms by targeted top-down proteomics termed Proteoform Reaction Monitoring (PfRM). We explore the ability of our method to circumvent traditional limitations of top-down proteomics such as sensitivity an...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2023-04-21 | MSV000091774 | MassIVE
ProteomicsProtein turnover measurement using selected reaction monitoring-mass spectrometry (SRM-MS) C4PR_LIV
Protein turnover represents an important mechanism in the functioning of cells, with deregulated synthesis and degradation of proteins implicated in many diseased states. Therefore, proteomics strategies to measure, with high confidence, turnover rates are of vital importance to understanding many ...
ORGANISM(S): Mus musculus (Mouse) 
2016-08-10 | PXD004413 | Pride
Srm Proteomics Ms1 Mass spectrometry Protein turnover Quantification 
ProteomicsMultiplex selected reaction monitoring assay for quantification of 15 human tissue kallikreins in biological samples
Human tissue kallikreins (KLKs) are a group of 15 secreted serine proteases encoded by the largest contiguous cluster of protease genes in the human genome. KLKs are involved in coordination of numerous physiological functions including regulation of blood pressure, neuronal plasticity, skin desquam...
ORGANISM(S): Homo sapiens (Human) 
2016-07-08 | PXD003324 | Pride
Cervicovaginal fluid Sweat Klk4 secretion Mass spectrometry Seminal plasma Selected reaction monitoring Kallikreins 
ProteomicsAbsolute protein quantification of Mycoplasma pneumoniae proteome by combining selected reaction monitoring of a reduced set of labeled peptides and label-free shotgun mass spectrometry.
We quantified the protein abundances of Mycoplasma pneumoniae (Mpn), a genome-reduced organism, by combining selected reaction monitoring of a reduced set of labeled peptides and label-free shotgun mass spectrometry. A set of 77 labeled peptides were synthesized and used as internal standard peptide...
ORGANISM(S): Mycoplasma pneumoniae (strain ATCC 29342 / M129) 
2023-08-31 | PXD035159 | Pride
Mycoplasma pneumoniae Absolute quantification Label-free Selected reaction monitoring 
ProteomicsParallel Reaction Monitoring of 42 peptide pairs with 15N metabolic labeling
To examine the properties of 14N and 15N labeled peptides from 15 metabolic labeling samples, we set up targeted quantification using Parallel Reaction Monitoring (PRM) on a high resolution, high accuracy mass spectrometer. We first examined the co-elution of light and heavy peptide forms. Furthermo...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2022-06-09 | PXD030112 | Pride
Arabidopsis Quantification 15n metabolic labeling Protein prospector Targeted quantification Parallel reaction monitoring (prm) 
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