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ProteomicsSequential Phosphoproteomic Enrichment through Complementary Metal-Directed Immobilized Metal Ion Affinity Chromatography
We developed a tip-based sequential IMAC based on different kinds of metal ion for increasing the coverage of phosphoproteome. This approach not only can be used for singly and multiply phosphopeptides separation but also for acidic, basophilic and Pro-directed phosphopeptides separation.
ORGANISM(S): Homo Sapiens (human) 
2016-09-07 | PXD004903 | JPOST Repository
ProteomicsSequential Phosphoproteomic Enrichment by Complementary Metal-directed Immobilized Metal Ion Affinity Chromatography: Case Study on Kinase Substrate Mapping in Human Lung Cancer Tissue
Abstract: Despite recent advances in instrumentation and analytical strategies for identification and quantitation of protein phosphorylation, methodologies to enrich the heterogeneous types of phosphopeptides are critical towards comprehensive mapping of the under-explored phosphoproteome. Taking a...
ORGANISM(S): Homo sapiens (Human) 
2014-01-29 | PXD000314 | Pride
Human lung cancer tissue Sequential imac Phosphoproteomics 
ProteomicsIntegrated Single-Tip IMAC-HILIC Enrichment Enables Simultaneous and Efficient Analysis of Plant Phosphoproteomics and N-glycoproteomics
Protein phosphorylation and N-glycosylation are key post-translational modifications (PTMs) in plants that regulate critical signaling processes. However, analyzing both PTMs simultaneously remains challenging due to low abundance and divergent enrichment requirements. Here, we present a single-tip ...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-05-14 | PXD061247 | Pride
Phosphoproteomics  n-glycoproteomics  enrichment  immobilized metal affinity chromatography  hydrophilic interaction chromatography 
UnknownTIMAHAC: Streamlined Tandem IMAC-HILIC Workflow for Simultaneous and High-Throughput Plant Phosphoproteomics and N-glycoproteomics.
Not available
S-EPMC11098956 | biostudies-literature
ProteomicsSystematic evaluation of experimental parameters for sensitive phosphoproteomics analysis
The field of phosphoproteomics has been revolutionized in the last years, with enhanced efficiency and sensitivity, now enabling deep phosphoproteomic analysis single-shot from minimal peptide inputs. In this study, we rigorously evaluate the effects of various experimental parameters in automated Z...
ORGANISM(S): Homo sapiens (Human) 
2024-03-26 | PXD045601 | Pride
Phosphoproteomics Evosep Sequential enrichment 
ProteomicsHs_Lung_Phospho_SequentialIMAC_tissue
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Cancer tissues
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005323 | PeptideAtlas
ProteomicsHs_Lung_Phospho_SequentialIMAC_cell
Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Raji cell line
ORGANISM(S): Homo Sapiens (human) 
2014-12-31 | PAe005320 | PeptideAtlas
ProteomicsPhosphoproteomic analysis of human neutrophil granule exocytosis primed by TNF
Human neutrophil phosphopeptide study using sequential TiO2 and IMAC isolation
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-06-30 | MSV000079871 | MassIVE
ProteomicsLarge scale phosphoprotein profiling to unravel Drosophila cold acclimation regulatory mechanisms
In this study we compared the phosphoproteome of control and cold acclimated Drosophila melanogaster in order to unravel the cold acclimation regulation mechanisms. For this prurpose we enriched phosphopeptides from each sample using a sequential elution from IMAC strategy (SIMAC) and analyzed them ...
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2017-05-16 | PXD005311 | Pride
Acclimation Simac Fruit fly Phosphorylation network 
UnknownEvaluation of quantitative performance of sequential immobilized metal affinity chromatographic enrichment for phosphopeptides.
Not available
S-EPMC3909987 | biostudies-literature
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