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ProteomicsEvosep One Enables Robust Quantitative Deep Proteome Coverage using Tandem Mass Tags while Significantly Reducing Machine Time
The balance between comprehensively analyzing the proteome and using valuable mass spectrometry time is a genuine challenge in the field of proteomics. Multidimensional fractionation strategies have significantly increased proteome coverage, but often at the cost of increased mass analysis time, des...
ORGANISM(S): Homo sapiens (Human) 
2019-04-04 | PXD012520 | Pride
Evosep Nonsmall cell lung carcinoma (nsclc) patient-derived xenografts (pdx) Tmt Mass analysis time Lc-ms/ms 
UnknownOptimizing Proximity Proteomics on the EvoSep-timsTOF LC-MS System.
Not available
S-EPMC12329395 | biostudies-literature
ProteomicsOptimised Sample Handling Minimises Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics
Chemical proteomics approaches often yield low peptide recovery because they aim to enrich low-abundance proteins. Poor sample handling further reduces recovery through peptide adsorption to plastic surfaces and losses due to vacuum evaporation. We systematically mapped these losses across buffers, ...
ORGANISM(S): Homo sapiens (Human) 
2026-06-23 | PXD069322 | Pride
Evosep Optimisation Chemoproteomics 
ProteomicsEvoSep-timsTOF proximity dependent biotinylation optimization
This dataset involved the optimization of proximity dependent biotinylation experiments across the available EvoSep gradients on a Bruker timsTOF Pro 2 MS. Various different sample loads are compared and statistically significant preys are used to select optimal settings for these kinds of experimen...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2024-07-09 | MSV000095270 | MassIVE
UnknownA Novel LC System Embeds Analytes in Pre-formed Gradients for Rapid, Ultra-robust Proteomics.
Not available
S-EPMC6210218 | biostudies-literature
ProteomicsFelipe Sheep EV DIA LC/MS/MS using the Orbitrap Astral
DIA analysis of sheep EVs using Evosep one and Orbitrap Astral
ORGANISM(S): Ovis aries 
2026-09-14 | PXD077206 | Pride
Ev Dia Astral Sheep 
UnknownFully Automated Workflow for Integrated Sample Digestion and Evotip Loading Enabling High-Throughput Clinical Proteomics.
Not available
S-EPMC11251069 | biostudies-literature
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054105 | Pride
Data independant acquistion Label free quantification 
UnknownDistinct Urinary Proteome Changes Across Estimated Glomerular Filtration Rate Stages in a Cohort of Black South Africans.
Not available
S-EPMC11855517 | biostudies-literature
ProteomicsRobust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - Library Build, concentration response, structure activity relationships and in-cell treatment
In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. Within this workflow, we profile cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiob...
ORGANISM(S): Homo sapiens (Human) 
2024-11-18 | PXD054145 | Pride
Chemoproteomics. lavel-free quantification Data independant acquisition 
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