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Synchronized C. elegans cultures were prepared using standard techniques (http://cmgm.stanford.edu/~kimlab/index_methods.html). Live young adult worms were split between NG plates pre-seeded with the non-pathogenic E. coli strain OP50 or the Pseudomonas aeruginosa clinical isolate PA14 and incubated...
ORGANISM(S): Caenorhabditis elegans 
Oxidative stress caused by Menadione or Hydrogen peroxide in synchronized Saccharomyces cerevisiae cultures. Alpha factor synchronized cultures (0.2-0.4 OD), treated at the beginning of S phase (25 min after release from G1 arrest) with either 2 mM Menadione (MD) or 0.24 mM Hydrogen peroxide (HP), s...
ORGANISM(S): Saccharomyces cerevisiae 
Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark). Three conditions (E.coli, P. aeurginosa colonized, and P. aeurginosa non-...
ORGANISM(S): Caenorhabditis elegans 
Stress experiments repeating Gasch et al.,2000 conditions (Mol Biol Cell. 2000 Dec;11(12):4241-57). For each set of conditions I compared a deletion mutant for a predicted transcription regulator with a congenic wt strain. Groups of assays that are related as part of a time series. Computed
ORGANISM(S): Saccharomyces cerevisiae 
Stress experiments repeating Gasch et al.,2000 conditions. For each set of conditions I compared a deletion mutant for a predicted transcription regulator with a congenic wt strain.
ORGANISM(S): Saccharomyces cerevisiae 
Transcriptional profiling of adult C.elegans exposed to E.coli or to GFP-expressing P. aeruginosa (strain PA14). For P. aeruginosa exposure, worms were separated into 2 groups - fully colonized (green) or non-colonized (dark). Six conditions ( control or elt-2 RNAi ; E.coli, P. aeurginosa colonized,...
ORGANISM(S): Caenorhabditis elegans 
Insults to the cerebral cortex, such as trauma, ischemia or infections, may result in the development of epilepsy, one of the most common neurological disorders. Previous studies have suggested that perturbations in neurovascular integrity and breakdown of the blood-brain barrier (BBB) lead to neuro...
ORGANISM(S): Rattus norvegicus 
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