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ProteomicsTMTpro-18plex analysis of MM1.S cells treated with thalidomide and its derivatives
MM1.S cells were treated with DMSO or 10 µM thalidomide or its derivatives for 5 h in two biological replicates. The cells were lysed in 200 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonication, proteins (100 µg each) wer...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD041812 | JPOST Repository
ProteomicsLFQ of biotinylated peptides from THP-1 cells expressing AirID-CRBN
THP-1 cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, or 10 µM pomalidomide in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by L...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028760 | JPOST Repository
ProteomicsLFQ of biotinylated peptides from IMR32 cells expressing AirID-CRBN
IMR32 cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide, or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated peptide...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028758 | JPOST Repository
ProteomicsLFQ of biotinylated peptide from HuH7 cells expressing AirID-CRBN
HuH7 cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide, or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated peptides...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028757 | JPOST Repository
ProteomicsLFQ of biotinylated peptides from HEK293T cells expressing AirID-CRBN
HEK293T cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide, or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated pepti...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028756 | JPOST Repository
ProteomicsNeo-substrate selectivity of IMiDs on biotinylation by AirID-CRBN
MM1.S cells stably expressing AGIA-AirID-CRBN-WT were treated with DMSO, 20 µM thalidomide, 10 µM lenalidomide, 10 µM pomalidomide or 20 µM 5-hydroxythalidomide (5HT) in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. After tryptic digestion, biotinylated peptides...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028755 | JPOST Repository
ProteomicsTMTpro-18plex analysis of MM1.S cells treated with PROTACs
MM1.S cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonicat...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037178 | JPOST Repository
ProteomicsTMTpro-18plex analysis of NTERA-2 cells treated with PROTACs
NTERA-2 cells were treated with DMSO, 0.3 µM Po-P, 0.3 µM Le-P, 0.3 µM F-P, 0.3 µM Cl-P, or 0.3 µM F3C-P for 16 h in three biological replicates. The cells were lysed in 100 µL guanidine buffer (6 M guanidine-HCl, 100 mM HEPES-NaOH, pH 7.5, 10 mM TCEP, 40 mM chloroacetamide). After heating and sonic...
ORGANISM(S): Homo Sapiens (human) 
2023-07-22 | PXD037179 | JPOST Repository
ProteomicsComparison of three enrichment methods of biotinylated peptides or proteins from AirID-CRBN-expressing MM1.S cells
MM1.S cells stably expressing AGIA-AirID-CRBN were treated with DMSO or 10 µM pomalidomide in the presence of 10 µM biotin and 5 µM MG132 for 8 h in three biological replicates. The cells were divided into three tubes and lysed. After tryptic digestion, biotinylated peptides were enriched using Tama...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028754 | JPOST Repository
ProteomicsLFQ of indisulam-dependent biotinylated peptides from HCT116 cells expressing AirID-DCAF15
HCT116 cells stably expressing AGIA-AirID-DCAF15-WT or -D475N mutant were treated with DMSO or 5 µM indisulam in the presence of 50 µM biotin and 10 µM MG132 for 6 h in three biological replicates. After tryptic digestion, biotinylated peptides were enriched with Tamavidin 2-REV and analyzed by LC-M...
ORGANISM(S): Homo Sapiens (human) 
2021-11-06 | PXD028762 | JPOST Repository
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