TroponinT was incubated with calpain. Incubation time and labels are described in the sample information.
Triplicated LC-MS data were merged and processed with ProteinPilot (ver5).
ORGANISM(S): Homo Sapiens (human) Cellular Organisms
Comparative analysis of soluble proteomes in skeletal muscle from calpain-3 knock-out mouse or proteolytically inactive calpain-3 knock-in mouse to that of wild type mouse.
HEK293T cells were transiently transfected with plasmids encoding Maltose binding protein (MBP)-tagged proteolytically inactive CAPN (CAPN3:CS) and constitutively active form of Fyn (Fyn-YF). CAPN3:CS was pulldown from the HEK293T cell lysates using amylose-resin, purified as an excised band from SD...
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. We isolate...
CARASIL (cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy) is a hereditary cerebral small-vessel disease caused by loss of function mutations of HTRA1, which is a serine protease with a variety of targets, including extracellular matrix proteins. Htra1-KO m...
