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Proteomicsshotgun LC/MS/MS of L(3)mbt-IP samples using OSCs
L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed shotgun LC/MS/MS of L(3)mbt-IP samples and identified a new interactor in OSCs.
ORGANISM(S): Drosophila melanogaster (Fruit fly) 
2022-08-15 | PXD026945 | Pride
Osc Drosophila 
TranscriptomicsCloning of 50-75 nt small RNAs associated with Armitage in OSCs
Armitage (Armi) is a component of Yb bodies and functions in the primary piRNA processing pathway. Armi interaction with Piwi does not require Yb expression; however, without Yb, the Armi-Piwi complex does not localize at Yb bodies and contains few piR-ILs (small RNAs associated with Armitage compl...
ORGANISM(S): Drosophila melanogaster 
2010-10-24 | E-GEOD-23931 | biostudies-arrayexpress
ProteomicsDIA-PASEF analysis of Shu immunoprecipitates from control and Shu knockdown Drosophila OSCs
After control or Shu knockdown (n = 3 each), OSCs were lysed in binding buffer [50 mM Tris-HCl (pH 8.0), 150 mM KOAc, 5 mM Mg(OAc)2, 5 mM DTT, 0.1% NP-40, 2 µg/mL leupeptin, 2 µg/mL pepstatin A, 0.5% aprotinin, 10% glycerol] and centrifuged to remove insoluble debris. The lysates were then incubated...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2026-02-04 | PXD074025 | JPOST Repository
ProteomicsDIA-PASEF analysis of FLAG immunoprecipitates from Shu-FLAG- and luciferase-FLAG-expressing Drosophila OSCs
After expression of Shu-FLAG or luciferase-FLAG (n = 3 each), OSCs were lysed in binding buffer [50 mM Tris-HCl (pH 8.0), 150 mM KOAc, 5 mM Mg(OAc)2, 5 mM DTT, 0.1% NP-40, 2 µg/mL leupeptin, 2 µg/mL pepstatin A, 0.5% aprotinin, 10% glycerol] and centrifuged to remove insoluble debris. The lysates we...
ORGANISM(S): Drosophila Melanogaster (fruit Fly) 
2026-02-04 | PXD074026 | JPOST Repository
ProteomicsPhosphorylation sites that influences the RNA-binding activity of Papi
We conducted UV cross-linking immunoprecipitation (CLIP) with and without phosphatase inhibitor and compared the outcome. The topmost band on the western blots appeared more prominent with the inhibitor. The RNA-binding activity of the band was also stronger with the inhibitor. We isolated the band ...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027395 | JPOST Repository
ProteomicsPapi interacting proteins in mitochondrial fraction of BmN4 cells
We incubated Papi-Flag in BmN4 mitochondrial lysates in the presence of 32P-ATP. Upon incubation, Papi-Flag was 32P-labeled, suggesting the presence of kinases for Papi in the fraction. Papi-Flag was immunoprecipitated after incubation in mitochondrial lysates under ATP-free conditions. The proteins...
ORGANISM(S): Bombyx Mori 
2022-02-01 | PXD027396 | JPOST Repository
ProteomicsAgo3 interaction proteins in BmN4 cell
We prepared BmN4 cell lysates in Co-IP buffer [30 mM HEPES-KOH pH 7.3, 150 mM potassium acetate, 5 mM magnesium acetate, 5 mM dithiothreitol, 0.1% (w/v) NP-40 and cOmplete ULTRA EDTA-free protease inhibitor (Roche)] with and without 1 mM EDTA and conducted immunoprecipitation assays using anti-Ago3...
ORGANISM(S): Bombyx Mori 
2021-01-08 | PXD023467 | JPOST Repository
TranscriptomicsFLX sequencing of Piwi-associated small RNAs extracted from Drosophila ovarian somatic cells (OSC)
piRNAs function in silencing retrotransposons by associating with the PIWI proteins, AGO3, Aub, and Piwi, in Drosophila germlines. Bioinformatics analyses of piRNAs in Drosophila ovaries suggested that piRNAs are produced by two systems, the primary processing pathway and the amplification loop, fro...
ORGANISM(S): Drosophila melanogaster 
2009-10-01 | E-GEOD-15137 | biostudies-arrayexpress
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