Sort   by:  
 Page size 
TranscriptomicsEndogenous siRNAs derived from transposons and mRNAs in Drosophila somatic cells
Small interfering RNAs (siRNAs) direct RNA interference (RNAi) in eukaryotes. In flies, somatic cells produce siRNAs from exogenous double-stranded RNA as a defense against viral infection. Here, we identify 21-nt long, endogenous siRNAs (endo-siRNAs) corresponding to transposons and heterochromatic...
ORGANISM(S): Drosophila melanogaster 
2008-04-10 | E-GEOD-11019 | biostudies-arrayexpress
GenomicsTranscription profiling by array of embryonic chick retina treated with Hes5.3 siRNAs, Atoh7 siRNAs and non-targeting siRNAs.
Transcription profiling by array of embryonic chick retina treated with Hes5.3 siRNAs, Atoh7 siRNAs and non-targeting siRNAs.
PRJNA613618 | ENA
ProteomicsTMT-MS of PCIF1 siRNAs treated RPE-1 cells
RPE-1 cells treated with the indicated siRNAs for 48 h were subjected to the tandem mass tag quantitative proteomics analysis.Carry out relative protein quantification analysis using tandem mass tag mass spectrometry in RPE-1 cells to investigate the mechanism underlying PCIF1 attenuates ciliation.
ORGANISM(S): Homo sapiens (Human) 
2024-03-21 | PXD044338 | Pride
Pcif1 Rpe-1 Tmt-ms 
TranscriptomicsProcessing of Drosophila endo-siRNAs depends on a specific Loquacious isoform
Drosophila melanogaster expresses three classes of small RNAs, which are classified according to their mechanisms of biogenesis. MicroRNAs are ~22-23-nt, ubiquitously expressed small RNAs that are sequentially processed from hairpin-like precursors by Drosha/Pasha and Dcr-1/Loquacious complexes. Mic...
ORGANISM(S): Drosophila melanogaster 
2009-07-30 | E-GEOD-17171 | biostudies-arrayexpress
TranscriptomicsmicroRNA-like off-target transcript regulation by siRNAs
siRNAs mediate sequence-specific gene silencing in cultured mammalian cells but also silence unintended transcripts. Many siRNA off-target transcripts match the guide-strand ‘‘seed region,’’ similar to the way microRNAs match their target sites. The extent to which this seed-matched, microRNA-like, ...
ORGANISM(S): Mus musculus 
2009-01-31 | E-GEOD-14073 | biostudies-arrayexpress
TranscriptomicsTissue specific expression of chalcone synthase siRNAs
In a previous study, seed coat and cotyledon tissues of Williams, Richland and T157 soybean lines were investigated to show tissue specificity of CHS siRNA expression (Tuteja et al., 2009). Here, we investigated more tissues such as leaf, root and germinating cotyledon to ascertain the tissue specif...
ORGANISM(S): Glycine max 
2014-02-13 | E-GEOD-49708 | biostudies-arrayexpress
TranscriptomicsIn depth sequencing of the siRNAs associated with peach latent mosaic viroid infection
Using a high-throughput sequencing approach we quantitatively analyzed the content of viroid-derived siRNAs of an infected tree. Our results show that the entire PLMVd genome is found in the siRNA population. Also both polarities are susceptible to be targeted by the RNAi machinery but specific regi...
ORGANISM(S): Prunus persica 
2010-02-20 | E-GEOD-18764 | biostudies-arrayexpress
TranscriptomicsOptimized siRNAs with a minimal off-target effect facilitate the screening of essential genes in cancer cells
siRNAs with dN are a promising and easy approach to characterize essential genes by minimizing the off-target effect in cancer cells. mRNA profiles of HCT 116 cells after the transfection of siRNAs or blank control by deep sequencing, using HiSeq 2000
ORGANISM(S): Homo sapiens 
2015-07-01 | E-GEOD-62968 | biostudies-arrayexpress
GenomicsVirus-derived siRNAs in Arabidopsis thaliana
Virus-derived siRNAs in Arabidopsis thaliana
PRJNA122617 | ENA
TranscriptomicsIdentification and analysis of transgene-derived endo-siRNAs in Drosophila Schneider cells by Solexa sequencing.
Colonization of genomes by a new selfish genetic element is detrimental to the host species and must lead to an efficient, repressive response. In vertebrates as well as in Drosophila, piRNAs repress transposons in the germ line while endogenous siRNAs take on this role in somatic cells. For endo-si...
ORGANISM(S): Drosophila melanogaster 
2009-09-13 | E-GEOD-16958 | biostudies-arrayexpress
Sort   by:  
 Page size