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ProteomicsEstablishment of plasmodesmata core proteome using label free semi-quantitative proteomic analysis.
To establish the plasmodesmata core proteome, the identification and the relative amount of proteins in different cellular fractions, namely, the plasmodesmata, plasma membrane, total cell extract, microsomal and cell wall fractions, were determined using label-free quantification method. Four to si...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2019-07-05 | PXD006800 | Pride
Arabidopsis thaliana Label-free Plasmodesmata Proteomics 
TranscriptomicsRNA-seq data from healthy and DMD human cells, along skeletal muscle differentiation from induced pluripotent stem cells
3 healthy cells - 3 DMD cells - 7 time points (tissue-derived myoblasts, tissue-derived myotubes, hiPSCs, Day 3 of differentiation, Day 10 of differentiation, Day 17 of differentiation, Day 25 of differentiation) - 1 replicate per cell line for the tissue-derived samples and 3 biological replicates ...
ORGANISM(S): Homo sapiens 
2020-10-05 | E-MTAB-8321 | biostudies-arrayexpress
TranscriptomicsmiRNA-seq data from healthy and DMD human cells, along skeletal muscle differentiation from induced pluripotent stem cells
3 or 4 healthy cells - 3 DMD cells - 7 time points (tissue-derived myoblasts, tissue-derived myotubes, hiPSCs, Day 3 of differentiation, Day 10 of differentiation, Day 17 of differentiation, Day 25 of differentiation).
ORGANISM(S): Homo sapiens 
2020-10-05 | E-MTAB-8293 | biostudies-arrayexpress
ProteomicsChanges in the nuclear proteome of developing wheat (Triticum aestivum L.) grain
Nuclear proteins of developing wheat grains collected during the cellularization, effective grain-filling and maturation phases of development were analysed.. Nuclear proteins were extracted and separated by two-dimensional gel electrophoresis. Image analysis revealed 371 and 299 reproducible spots ...
ORGANISM(S): Triticum aestivum (Wheat) 
2015-11-20 | PXD002999 | Pride
Developing grain Nuclear proteins Wheat Lc-ms/ms 
TranscriptomicsTranscription profiling of human tumor-conditioned versus quiescent HUVEC cells, and 5-aza-2a??-deoxycytidine and trichostatin AI treated cells to identify epigenetically silenced genes
Tumor angiogenesis requires intricate regulation of gene expression in endothelial cells (EC). We recently showed that DNA methyltransferase (DNMT)- and histone deacetylase (HDAC) inhibitors directly repress EC growth and tumor angiogenesis, suggesting that epigenetic modifications mediated by DNMTs...
ORGANISM(S): Homo sapiens 
2008-06-15 | E-GEOD-7132 | biostudies-arrayexpress
TranscriptomicsTranscription profiling of 57 hepato cellular carcinoma tumoral samples, 3 hepatocellular adenomas, 5 non-tumoral pools
A ""Cartes d'Identite des Tumeurs"" (CIT) project from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net). 65 samples on Affymetrix HG-U133A GeneChips arrays (57 tumoral samples, 3 hepatocellular adenomas, 5 non-tumoral pools). Identification of six subgroups of Hepatocellular...
ORGANISM(S): Homo sapiens 
2007-03-26 | E-TABM-36 | biostudies-arrayexpress
TranscriptomicsDetermining the impact of alternative splicing events on transcriptome dynamics
Background The complete sequencing of the human genome and its subsequent analysis revealed a predominant role for alternative splicing in the generation of proteome diversity. Splice switching oligonucleotides (SSOs) are a powerful and specific tool to experimentally control alternative splicing of...
ORGANISM(S): Homo sapiens 
2008-10-02 | E-GEOD-9851 | biostudies-arrayexpress
TranscriptomicsMicroarrays analysis of SREBP-1a and SREBP-1c target genes identify new regulatory pathways.
In this study we have identified the target genes of SREBP1a and SREBP1c in primary cultures of human skeletal muscle cells using adenoviral vectors expressing the mature nuclear form of human SREBP1a or SREBP1c combined with oligonucleotide microarrays. Keywords: comparison of human myotubes infec...
ORGANISM(S): Homo sapiens 
2010-05-26 | E-GEOD-10918 | biostudies-arrayexpress
TranscriptomicsFunctional and transcriptional heterogeneity of human hemopoietic lympho-myeloid progenitors
Cord blood (CB) samples from normal donors were obtained with informed consent. Fresh CB samples were processed within 18-34h after collection. Mononuclear cells were isolated and CD34+ fraction was separated. CB CD34+ enriched fraction was lineage depleted by staining with purified anti-human CD2, ...
ORGANISM(S): Homo sapiens 
2017-10-03 | E-MTAB-5456 | biostudies-arrayexpress
TranscriptomicsUp-regulation of IFN-related genes in BRCA2-/- cells
Microarray-based expression profiling of BRCA2 knockout and isogenic wild type HCT116 human colorectal cancer cells One way ANOVA, single factor comparison of wild type and BRCA2 knockout cells, three CEL files for wild type, three CEL files for BRCA2 knockout
ORGANISM(S): Homo sapiens 
2014-07-01 | E-GEOD-54830 | biostudies-arrayexpress
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