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Proteomicsdichotomic size-exclusion chromatography（SEC）
directDIA proteomics raw data of plasma extracellular vesicles isolated by SEC and UC
ORGANISM(S): Homo Sapiens 
PXD026034 |
ProteomicsGlobal profiling of the RNA and protein complexes of Escherichia coli by size exclusion chromatography followed by RNA sequencing and mass spectrometry (SEC-seq)
21 total proteome samples from E. coli MG1655 wild type fractionated by size exclusion chromatography resulting in 20 fractions. A lysate sample is given as input control.
ORGANISM(S): Escherichia coli 
2022-11-15 | PXD036475 | Pride
Size exclusion chromatography E. coli Lc-ms/ms 
ProteomicsSystem-wide profiling of protein-complexes via size exclusion chromatography– mass spectrometry (SEC-MS)
In living cells most proteins are organized in stable or transient functional assemblies known as protein complexes, which control a multitude of vital cellular processes such as cell cycle progression, metabolism, and signal transduction. System-wide workflows for analysis of protein complexes usin...
ORGANISM(S): Homo sapiens (Human) 
2022-05-06 | PXD018033 | Pride
Protein complex analysis Sec-ms Protein co-elution profiling 
ProteomicsTop-down Proteomics of Large Proteins Up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy
Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and post-translational modifications (PTMs). However, top-down MS analysis of high molecular weight (MW) proteins remains challenging mainly due to t...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2016-11-29 | MSV000080365 | MassIVE
ProteomicsSEC-SWATH-MS HEK293 - Complex-centric proteome profiling by SEC-SWATH-MS
Increasingly, biochemical co-fractionation-based approaches are used to study interactomes and protein complexes at high throughput. The devised methods facilitate the qualitative assignment and prediction of hundreds of putative cellular assemblies in one experiment and without dependency on geneti...
ORGANISM(S): Homo sapiens (Human) 
2019-01-11 | PXD007038 | Pride
Size exclusion chromatography Mass spectrometry Swath Protein complexes 
GenomicsGlobal profiling of the RNA and protein complexes of Escherichia coli by size exclusion chromatography followed by RNA sequencing and mass spectrometry (SEC-seq)
Global profiling of the RNA and protein complexes of Escherichia coli by size exclusion chromatography followed by RNA sequencing and mass spectrometry (SEC-seq)
PRJNA875378 | ENA
ProteomicsLeveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size-exclusion Chromatography for Proteome-wide Crosslinking Mass Spectrometry_CLMS dataset
Ion mobility spectrometry shows great promise to tackle analytically challenging research questions by adding another separation dimension to liquid chromatography-mass spectrometry. The understanding of how analyte properties influence ion mobility has increased through recent studies but no clear ...
ORGANISM(S): Homo Sapiens (human) Cellular Organisms 
2022-12-16 | PXD022360 | JPOST Repository
ProteomicsLeveraging Parameter Dependencies in High-Field Asymmetric Waveform Ion-Mobility Spectrometry and Size-exclusion Chromatography for Proteome-wide Crosslinking Mass Spectrometry_protein ID dataset
Ion mobility spectrometry shows great promise to tackle analytically challenging research questions by adding another separation dimension to liquid chromatography-mass spectrometry. The understanding of how analyte properties influence ion mobility has increased through recent studies but no clear ...
ORGANISM(S): Homo Sapiens (human) 
2022-12-16 | PXD022341 | JPOST Repository
ProteomicsSelf-packed size-exclusion columns enable versatile high-throughput native, top-down, and ion-mobility-mass spectrometry studies on proteins and complexes.
Native MS (nMS) is a key structural biology technique that makes it possible to study intact proteins and their interactions. Unfortunately, non-volatile salts are incompatiblewith nMS, which demands a laborious desalting procedure. Non-denaturing size exclusion chromatography (SEC) allows both rapi...
ORGANISM(S): Bos taurus (Bovine) Candida albicans (Yeast) Homo sapiens (Human) Escherichia coli Gallus gallus (Chicken) Equus caballus (Horse) 
2025-06-29 | PXD061452 | Pride
Ligand screening Top-down proteomics Native mass spectrometry Size-exclusion chromatography Protac Ion mobility 
ProteomicsExtensive heterogeneity of glycopeptides in plasma revealed by deep glycoproteomic analysis
A number of plasma glycoproteins are clinically useful as biomarkers in a variety of diseases. Although thousands of proteins are present in plasma, >95% of the plasma proteome by mass is represented by only 22 proteins. This necessitates strategies to deplete the abundant proteins and enrich other...
ORGANISM(S): Homo sapiens (Human) 
2021-08-18 | PXD025414 | Pride
N-glycoproteomics Size exclusion chromatography Plasma glycoproteomics 
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