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Both SKOV3 and IOSE80 cells were cultured and extracted the proteins. With sequential digestion by trypsin, enriched by Zwitterionic Hydrophilic Interaction chromatography (ZIC-HILIC), the intact N-glycopeptides were then isotopic diethyl labelled and analyzed using C18-RPLC-MS/MS (HCD).
ORGANISM(S): Homo sapiens (Human) 
2023-03-10 | PXD030927 | Pride
RNAseq of SKOV3(AA) and SKOV3(CC)
EZH2 and H3K27ac ChIPseq of SKOV3(AA) and SKOV3(CC)
H3K27ac, H3K4me3, H3K27me3 and H3K9me3 ChIPseq of SKOV3(AA) and SKOV3(CC)
Next Generation Sequencing of Transcriptomes in SKOV3 and rhCCL20-treated SKOV3 cells
Expression data from SKOV3 KO1#_Vector and SKOV3 KO1#_MED12 rescued cells
To dissect the molecular mechanisms of PEA-15-mediated paclitaxel sensitization in ovarian cancer cells, we performed cDNA microarray analysis using SKOV3.ip1-S116A cells (Ser116 of PEA-15 substituted with alanine) and SKOV3.ip1-S116D cells (Ser116 of PEA-15 substituted with aspartic acid). cDNA mic...
ORGANISM(S): Homo sapiens 
Sequencing in transcriptomes in Ovarian cancer cell line SKOV3 and bevacizumab-treated SKOV3
LncRNA/mRNA expression in SKOV3/SKOV3_DDP/IOSE80
To identify genes whose expression was suppressed by EZH2, we performed gene expression microarray analysis in control and EZH2 knockdown human SKOV3 EOC cells. Two individual short hairpin RNAs to the human EZH2 gene (shEZH2) were used to limit potential off-target effects. Two individual short hai...
ORGANISM(S): Homo sapiens 
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