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GenomicsThe SPOC domain is a phosphoserine binding module that bridges transcription machinery with co- and post-transcriptional regulators - ChIP-seq
The heptarepeats of the C-terminal domain of Pol II are extensively modified throughout the transcription cycle. The CTD coordinates RNA synthesis and processing by recruiting transcription regulation factors as well as RNA capping, splicing and 3’end processing factors. The SPOC domain of PHF3 was ...
ORGANISM(S): Homo sapiens 
2023-02-22 | E-MTAB-11506 | biostudies-arrayexpress
ProteomicsAnti-FLAG co-immunoprecipitation of FLAG-tagged DIDO isoforms and mutants in HEK293T cells
The analysis of interactome of different DIDO isoforms and mutants upon transient overexpression of FLAG-tagged constructs in HEK293T cells.
ORGANISM(S): Homo sapiens (Human) 
2023-10-25 | PXD039540 | Pride
Dido Co-immunoprecipitation 
ProteomicsAnti-Pol II pS5 co-immunoprecipitation in PHF3 WT, KO and ΔSPOC HEK293T cells
The analysis of differential interactome of Pol II pS5 in PHF3 WT, KO and ΔSPOC HEK293T cells using Pol II pS5 (4H8) antibody coupled to Protein G beads.
ORGANISM(S): Homo sapiens (Human) 
2023-10-25 | PXD039537 | Pride
Rna pol ii ps5 Co-immunoprecipitation Phf3 
ProteomicsAnti-Pol II pS5 co-immunoprecipitation in DIDO WT, KO and ΔSPOC HEK293T cells
The analysis of differential interactome of Pol II pS5 in DIDO WT, KO and ΔSPOC HEK293T cells using Pol II pS5 (3E8) antibody coupled to Protein G beads (no crosslinking).
ORGANISM(S): Homo sapiens (Human) 
2023-10-25 | PXD039567 | Pride
Rna pol ii ps5 Dido Co-immunoprecipitation 
ProteomicsFLAG-PHF3 interactome in HEK293 cells
FLAG-hPHF3 was transiently expressed in human HEK293 cells, followed by anti-FLAG immunoprecipitation, on-bead digest and NanoLC-MS analysis.
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026292 | Pride
Human Anti-flag immunoprecipitation Nanolc-ms analysis On-bead digest 
TranscriptomicsPHF3 binds RNA polymerase II via the SPOC domain and regulates transcription of neuronal genes - PROseq
PRO-seq data of WT, KO, and dSPOC HEK293 cells.
ORGANISM(S): Homo sapiens 
2021-09-07 | E-MTAB-7501 | biostudies-arrayexpress
TranscriptomicsPHF3 regulates RNA stability through PHD and TLD domains - ChIPseq CNOT
Transcription and RNA processing are tightly coupled and precisely coordinated to ensure appropriate levels of mature transcripts. The C-terminal domain (CTD) of RNA polymerase II (Pol II) is phosphorylated differentially during the transcription cycle and serves as a landing pad for a variety of tr...
ORGANISM(S): Homo sapiens 
2025-06-13 | E-MTAB-13125 | biostudies-arrayexpress
TranscriptomicsPHF3 regulates RNA stability through PHD and TLD domains - RNAseq PHD - TLD
Transcription and RNA processing are tightly coupled and precisely coordinated to ensure appropriate levels of mature transcripts. The C-terminal domain (CTD) of RNA polymerase II (Pol II) is phosphorylated differentially during the transcription cycle and serves as a landing pad for a variety of tr...
ORGANISM(S): Homo sapiens 
2025-12-04 | E-MTAB-14530 | biostudies-arrayexpress
TranscriptomicsPHF3 regulates RNA stability through PHD and TLD domains - m6A
Transcription and RNA processing are tightly coupled and precisely coordinated to ensure appropriate levels of mature transcripts. The C-terminal domain (CTD) of RNA polymerase II (Pol II) is phosphorylated differentially during the transcription cycle and serves as a landing pad for a variety of tr...
ORGANISM(S): Homo sapiens 
2025-12-03 | E-MTAB-14529 | biostudies-arrayexpress
TranscriptomicsThe SPOC proteins DIDO3 and PHF3 co-regulate gene expression and neuronal differentiation - 3' RNA-seq
Transcription is regulated by a multitude of activators and repressors, which bind to the RNA polymerase II (Pol II) machinery and modulate its progression. Death-inducer obliterator (DIDO) and PHD finger protein 3 (PHF3) are paralogue proteins that regulate transcription elongation by docking onto ...
ORGANISM(S): Homo sapiens 
2023-10-31 | E-MTAB-12757 | biostudies-arrayexpress
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