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TranscriptomicsNext generation sequencing of small RNAs isolated from exosomes in human semen
Preparation of exosomes isolated from semen contain a substantial amount of RNA, mostly from 20 to 100 nucleotides in length. We sequenced separately 20-40 and 40-100 nucleotide fractions of RNA from exosomes isolated from semenal fluid from six healthy donors. We found various classes of small non...
ORGANISM(S): Homo sapiens 
2014-06-04 | E-GEOD-56076 | biostudies-arrayexpress
TranscriptomicsGlobal identification and characterization of intermediate-size non-coding RNAs in Oryza sativa
In this study, we employed a special size fractionation and cDNA library construction method followed by 454 deep sequencing to systematically profile rice intermediate-size ncRNAs. Our analysis resulted in the identification of 1349 ncRNAs in total, including 754 novel ncRNAs of an unknown function...
ORGANISM(S): Oryza sativa Japonica Group 
2012-07-27 | E-GEOD-39695 | biostudies-arrayexpress
TranscriptomicsChanges in expression of small non-coding RNAs/microRNAs during aging in C. elegans
We report a significant number of miRNAs and other non-coding small RNAs show dramatic changes in expression during aging in C. elegans. RNAs were prepared from four diffrent timepoints during adulthood of C. elegans hermaphrodites, Day 0, 5, 8 and 12 post-L4 molt, and used for making cDNA libraries...
ORGANISM(S): Caenorhabditis elegans 
2009-12-31 | E-GEOD-18634 | biostudies-arrayexpress
TranscriptomicsThe non-coding RNA of the multidrug resistance-linked vault particle encodes multiple regulatory small RNAs
Vault particles are conserved organelles that have been implicated in multidrug resistance and intracellular transport. They are formed by three different proteins and the non-coding vault RNAs (vRNAs). Here we show that human vRNAs produce several small RNAs (svRNAs) by mechanisms different from th...
ORGANISM(S): Homo sapiens 
2009-09-07 | E-GEOD-16060 | biostudies-arrayexpress
TranscriptomicsGenome-wide profiling of miRNAs and other small non-coding RNAs in the Verticillium dahliae inoculated cotton roots
MicroRNAs (miRNAs) and small interfering RNAs (siRNAs) are short (19–25 nucleotides) non-coding RNA molecules that have large-scale regulatory effects on development and on stress responses in plants.The objective of this study is to investigate the transcriptional profile of miRNAs and other smal...
ORGANISM(S): Gossypium hirsutum 
2011-04-29 | E-GEOD-28236 | biostudies-arrayexpress
TranscriptomicsGenomic features and regulatory roles of intermediate-size non-coding RNAs in Arabidopsis
Recent advances in genome-wide techniques allowed the identification of thousands of non-coding RNAs with various sizes in eukaryotes, some of which have further been shown to serve important functions in many biological processes. However, in model plant Arabidopsis, novel intermediate-sized ncRNAs...
ORGANISM(S): Arabidopsis thaliana 
2014-01-20 | E-GEOD-44235 | biostudies-arrayexpress
ProteomicsComprehensive Identification of Soybean Long Non-coding RNAs Reveals a Subset of Small Peptide-Coding Transcripts
Long non-coding RNAs (lncRNAs) are defined as non-protein-coding transcripts that are at least 200 nucleotides long. They are known to play pivotal roles in regulating gene expression, especially during stress responses in plants. We used a large collection of in-house transcriptome data from variou...
ORGANISM(S): Glycine max 
2020-01-09 | PXD014553 | Pride
Long non-coding rna Proteomics Soybean Small peptide Transcriptomics 
TranscriptomicsThe expression of small RNAs after prg-1 mutation
Piwi-related Argonaute proteins play important roles in maintaining germline integrity and fertility and have been linked to a class of germline-enriched small RNAs termed piRNAs. Caenorhabditis elegans encodes two Piwi family proteins called PRG-1 and PRG-2, and PRG-1 interacts with the C. elegans ...
ORGANISM(S): Caenorhabditis elegans 
2014-06-09 | E-GEOD-56272 | biostudies-arrayexpress
TranscriptomicsFunctional diversity and convergence of small non-coding RNAs in male germ cell differentiation and fertilization (piRNA and endo-siRNAs)
The small non-coding RNAs (sncRNAs) are considered as postranscriptional key regulators of male germ cell development. In addition to microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs), other sncRNAs generated from small nucleolar RNAs (snoRNAs), tRNAs or rRNAs processing may also play important ...
ORGANISM(S): Mus musculus 
2015-03-26 | E-GEOD-59254 | biostudies-arrayexpress
TranscriptomicsGlobal analyses of endonucleolytic cleavage in mammals reveal expanded repertoires of cleavage-inducing small RNAs and their targets
In mammals, small RNAs are important players in post-transcriptional gene regulation. While their roles in mRNA destabilization and translational repression are well appreciated, their involvement in endonucleolytic cleavage of target RNAs is poorly understood. Very few microRNAs are known to guide ...
ORGANISM(S): Homo sapiens 
2016-03-16 | E-GEOD-68253 | biostudies-arrayexpress
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