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ProteomicsSmg6 interacting partners in mouse
Nonsense-mediated RNA decay (NMD) is a highly conserved and selective RNA turnover pathway that depends on the endonuclease SMG6. Here, we show that SMG6 is essential for male germ cell differentiation in mice. Germ-cell conditional knockout (cKO) of Smg6 induces extensive transcriptome misregulatio...
ORGANISM(S): Mus musculus (Mouse) 
2022-10-01 | PXD037090 | Pride
Smg7 Chromatoid body Mouse Smg6 
TranscriptomicsmRNA-Seq from control cells and two clones of Flp-In-T-REx-293 cells modified via CRISPR-Cas9 to knockout SMG7 in combination to knockdowns of control, SMG5, and SMG6
Mature mRNAs undergo quality control during translation that may lead to RNA degradation by triggering the nonsense mediated decay (NMD) pathway. Aberrant translation due to features such as the presence of a premature stop codon downstream on an exon-exon junction or an intron in the 3'UTR activate...
ORGANISM(S): Homo sapiens 
2021-05-07 | E-MTAB-9330 | biostudies-arrayexpress
TranscriptomicsTranscriptomics analysis of gene expression in normal and Smg6 deficient mouse ES cells
RNA was isolated from control and Smg6 deficient ES cells using the TRIzol (Invitrogen) reagent by following the company manual. For all samples the RNA integrity was checked using an Agilent Bioanalyzer 2100. All samples showed a RIN (RNA integrity number) of higher than 9. Approximately 2.5 M-BM-5...
ORGANISM(S): Mus musculus 
2013-08-14 | E-GEOD-49844 | biostudies-arrayexpress
GenomicsTotal RNA sequencing in neuroprogenitors after SMG6 deletion
Total RNA sequencing in neuroprogenitors after SMG6 deletion
PRJNA776901 | ENA
GenomicsQuantitative profiling of SMG6 cleavage sites in HEK293 cells
Quantitative profiling of SMG6 cleavage sites in HEK293 cells
PRJNA1233271 | ENA
TranscriptomicsDirect identification of endogenous SMG6 targets and a preferred motif spanning SMG6 cleavage sites by parallel analysis of RNA ends in human cells
In metazoans, the endoribonuclease SMG6 is thought to cleave many endogenous mRNAs targeted for nonsense-mediated mRNA decay (NMD). However, most evidence as to the identity of endogenous SMG6 substrates is indirect, and little is known about their cleavage sites. Here, we report the efficacy of an ...
ORGANISM(S): Homo sapiens 
2014-12-01 | GSE61398 | GEO
ProteomicsComposite SMG5-1 SMG6 PIN domain formation is essential for NMD
Nonsense-mediated mRNA decay (NMD) relies on the coordinated assembly and action of multiple protein factors. Degradation of target mRNAs begins with endonucleolytic cleavage near premature stop codons, but the mechanisms of endonuclease activation and regulation remain unclear. Using structural pre...
ORGANISM(S): Homo sapiens (Human) 
2026-01-30 | PXD072588 | Pride
Nonsense-mediated mrna decay 
GenomicsCircadian gene expression study of SMG6 and WT mouse livers
Circadian gene expression study of SMG6 and WT mouse livers
PRJNA861077 | ENA
UnknownCell Type-Specific Role of RNA Nuclease SMG6 in Neurogenesis.
Not available
S-EPMC8699217 | biostudies-literature
TranscriptomicsQuantitative profiling of SMG6 cleavage sites in HEK293 cells
The nonsense-mediated mRNA decay pathway targets specific mRNAs for degradation in a translation-dependent manner. NMD can induce RNA turnover via either the bulk mRNA deadenylation and decapping machinery or via the specialized SMG6 endonuclease. SMG6 is a magnesium-dependent nuclease, leading to t...
ORGANISM(S): Homo sapiens 
2026-06-18 | GSE291379 | GEO
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