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TranscriptomicsSuhB associates with Nus factors to facilitate 30S ribosome biogenesis in Escherichia coli
This experiment consists of two studies. The first where two independent biological replicates of MG1655, MG1655 thyA suhB::thyA and MG1655 thyA nusB::thyA were each grown in LB to mid-exponential phase. RNA-seq and associated data analysis were performed as described previously (Stringer et al., 20...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2016-01-20 | E-MTAB-4240 | biostudies-arrayexpress
TranscriptomicsChIP-seq of MG1655 wt, MG1655 ΔphoB, MG1655 Δhns, MG1655 ΔphoB+Δhns and MG1655 PhoB-FLAG strains grown in MOPS media with either low phosphate (0.2 mM) or high phosphate (1.32 mM)
We used ChIP-seq to map the binding of of C-terminally FLAG3-tagged PhoB to the Escherichia coli K-12 genome during growth in MOPS minimal media with low phosphate or high phosphate (0.2 mM or 1.32 mM, respectively). As a control, we performed ChIP-seq in an untagged strain. We also used ChIP-seq to...
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2020-07-12 | E-MTAB-9293 | biostudies-arrayexpress
TranscriptomicsRNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)
We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).
ORGANISM(S): Escherichia coli str. K-12 substr. MG1655 
2020-07-12 | E-MTAB-9292 | biostudies-arrayexpress
TranscriptomicsRNA-seq of MG1655 wt and MG1655 delta-phoB strains grown in MOPS media with low phosphate (0.2 mM)
We used RNA-seq to determine differences in RNA levels between wild-type E. coli K-12 and a ΔphoB mutant, each grown in MOPS media with low phosphate (0.2 mM).
ORGANISM(S): Escherichia coli O17 
2020-10-03 | E-MTAB-9591 | biostudies-arrayexpress
TranscriptomicsMapping the Regulatory Network for Salmonella Typhimurium Invasion
Using RNAseq, the goal of the study is to investigate transcription factor regulation of virulence genes in 14028s Salmonella Typhimurium grown in SPI-1-inducing conditions. Transcription factors were cloned into pBAD24. RNA levels were compared in 14028s Salmonella Typhimurium with the transcriptio...
ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium 
2015-08-17 | E-MTAB-3765 | biostudies-arrayexpress
TranscriptomicsGenome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
We investigated the effect that intragenic s54 binding sites have on transcripton elongation. In an rpoN deletion we have over-expressed rpoN from a plasmid (with vector only contol) and then looked at changes in transcription elongation around intragenic s54 binding sites by RNA-seq. We are also lo...
ORGANISM(S): Escherichia coli 
2015-08-06 | E-MTAB-3699 | biostudies-arrayexpress
GenomicsGenome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding
We investigated the genome-wide binding sites for sigma54 and RNA polymerase in wild-type Escherichia coli MG1655.
ORGANISM(S): Escherichia coli 
2015-08-06 | E-MTAB-3697 | biostudies-arrayexpress
UnknownAssociation of timing of menarche with depressive symptoms and depression in adolescence: Mendelian randomisation study.
Not available
S-EPMC5209630 | biostudies-literature
TranscriptomicsComparative genomic hybridization of Clostridium sporogenes and Clostridium botulinum strains
Clostridium botulinum is a heterogeneous Gram-positive species that comprises four genetically and physiologically distinct groups of bacteria that share the ability to produce botulinum neurotoxin, the most poisonous toxin known to man, and the causative agent of botulism, a severe disease of human...
ORGANISM(S): Clostridium botulinum 
2007-05-23 | E-TABM-264 | biostudies-arrayexpress
TranscriptomicsTranscriptome analysis reveals differential splicing events in IPF lung tissue
Objectives: Idiopathic pulmonary fibrosis (IPF) is a complex disease in which a multitude of proteins and networks are disrupted. Interrogation of genome-wide transcription through RNA sequencing (RNA-Seq) enables the determination of genes whose differential expression is most significant in IPF, a...
ORGANISM(S): Homo sapiens 
2014-03-27 | E-GEOD-52463 | biostudies-arrayexpress
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