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We investigated the effect of homogenization strategy and protein precipitation on downstream protein quantitation using multiple reaction monitoring mass spectrometry (MRM-MS). Our objective was to develop a workflow capable of processing disparate tissue types with high throughput, minimal variabi...
ORGANISM(S): Mus Musculus 
2021-01-24 | PXD019539 | panorama
We developed 6,927 quantitative multiple reaction monitoring mass spectrometry (MRM-MS) assays that measure 2,187 unique proteins in 20 mouse organs and tissues to enable system-level analysis of protein expression in mice. Targets for assay development were selected by untargeted MS analysis, and c...
ORGANISM(S): Mus Musculus 
2024-01-04 | PXD020930 | panorama
Temperature sensitive (TS) missense mutants have been foundational in the characterization essential gene function. However, an unbiased approach for the analysis of the biochemical and biophysical changes in TS missense mutants within the context of their functional proteomes is lacking. We applied...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-11-03 | PXD017222 | Pride
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