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ProteomicsThe COMA complex plays a role in positioning Ipl1 activity proximal to Cse4 nucleosomes
Kinetochores are macromolecular protein complexes that ensure accurate chromosome segregation by linking chromosomes to spindle microtubules and integrating safeguard mechanisms. In yeast, the inner kinetochore, also known as Constitutive Centromere Associated Network (CCAN), is specifically establi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-04-12 | PXD011236 | Pride
Kinetochore  centromere  mtw  mif2  cross-linking  yeast 
ProteomicsAOCM-CPC - Kinetochores are macromolecular protein complexes that ensure accurate chromosome segregation by linking chromosomes to spindle microtubules and integrating safeguard mechanisms
Kinetochores are macromolecular protein complexes that ensure accurate chromosome segregation by linking chromosomes to spindle microtubules and integrating safeguard mechanisms. In yeast, the inner kinetochore, also known as Constitutive Centromere Associated Network (CCAN), is specifically establi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2019-04-12 | PXD011235 | Pride
Kinetochore  cpc  coma  cross-linking 
ProteomicsHistone modifications associated with the histone chaperone DAXX
A multitude of histone chaperones is required to protect histones from their biosynthesis to DNA deposition. They cooperate through the formation of co-chaperone complexes, but the crosstalk between nucleosome assembly pathways is unclear. Using explorative interactomics approaches, we map the organ...
ORGANISM(S): Mus musculus (Mouse) 
2023-03-06 | PXD034924 | Pride
Daxx Ptms H3.3 Histones 
ProteomicsQuantification of H3K27 methylation using heavy peptides as standards
To demonstrate the usability of our SIL peptides (JPT GmbH)on an MS application, 4 standard peptides were synthesized at 95% purity and each spiked at an amount 133 fmol into protein extracts of wild type HEK cells and EZH2-knockout cells, respectively. The spiked peptides in this experiment are his...
ORGANISM(S): Homo sapiens (Human) 
2020-04-23 | PXD018321 | Pride
Human Hek cells Histones modifications 
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