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TranscriptomicsSpo11-oligo mapping in S. cerevisiae Ctf19/CCAN kinetochore sub-complex mutant mcm21
During meiosis, crossover recombination is essential to link homologous chromosomes and drive faithful chromosome segregation. Crossover recombination is non-random across the genome, and centromere-proximal crossovers are associated with an increased risk of aneuploidy, including Trisomy 21 in huma...
ORGANISM(S): Saccharomyces cerevisiae 
2015-12-15 | E-GEOD-72683 | biostudies-arrayexpress
ProteomicsSpodoptera frugiperda kinetochore co-immunoprecipitations
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3, which physically interacts with components of the inner kinetochore constitutive-centromere-associated-network...
ORGANISM(S): Spodoptera frugiperda Bombyx mori (Silk moth) 
2020-02-18 | PXD016092 | Pride
Bombix Kinetochore Frugiperda Spodoptera 
TranscriptomicsPcs1 and Mde4 are inner centromere proteins required to prevent merotelic kinetochore orientation
Chromosome segregation depends on proper attachment of sister kinetochores to microtubules. Merotelic kinetochore orientation is an error which occurs when a single kinetochore is attached to microtubules emanating form opposite poles. Mechanisms preventing or correcting the merotelic attachment mus...
ORGANISM(S): Schizosaccharomyces pombe 
2010-06-10 | E-GEOD-5257 | biostudies-arrayexpress
ProteomicsThe composition and structure of the outer kinetochore KMN complex is conserved across Kingdoms
In eukaryotes, chromosome segregation during cell division requires attachment to the spindle, which is ensured by a large multi-protein structure called the kinetochore. The outer layer of the kinetochore attaches to the microtubules and is named after three of its components KNL1C, MIS12C, and NDC...
ORGANISM(S): Arabidopsis thaliana (Mouse-ear cress) 
2025-11-17 | PXD062209 | Pride
Ap-ms Arabidopsis Kinetochore Kmn complex 
ProteomicsBridgin connects the outer kinetochore to centromeric chromatin
In this project, 3XFlag-tagged protein affinity purifications were performed to identify the protein-protein interactors using Cryptococcus neoformans cell lysate. After fractionation with SDS-PAGE, peptide samples for nanoLC-MS/MS were prepared by in-gel digestion. Raw data from Orbotrap Velos pr...
ORGANISM(S): Cryptococcus Neoformans 
2020-09-23 | PXD021072 | JPOST Repository
GenomicsCentromere-Like Regions in Budding Yeast
Accurate chromosome segregation requires centromeres (CENs), the chromosomal sites where kinetochores form, to bridge DNA and attach to microtubules. In contrast to most eukaryotes, Saccharomyces cerevisiae possesses sequence-defined point centromeres. Chromatin immunoprecipitation followed by seq...
ORGANISM(S): Saccharomyces cerevisiae 
2013-01-18 | E-GEOD-31466 | biostudies-arrayexpress
TranscriptomicsExpression profiles of histone point mutants
Accurate chromosome segregation requires that sister kinetochores biorient and attach to microtubules from opposite poles. Kinetochore biorientation relies on the underlying centromeric chromatin, which provides a platform to assemble the kinetochore and to recruit the regulatory factors that ensur...
ORGANISM(S): Saccharomyces cerevisiae 
2013-12-04 | E-GEOD-39903 | biostudies-arrayexpress
TranscriptomicsDistribution of Arp4 in G2/M phase
The actin-related proteins (ARPs) comprise a conserved protein family. Arp4p is found in large multisubunits of the INO80 and SWR1 chromatin remodeling complexes and in the NuA4 histone acetyltransferase complex. Here we show that arp4 (arp4S23AD159A) temperature-sensitive cells are defective in G2/...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-11 | E-GEOD-7276 | biostudies-arrayexpress
ProteomicsThe stoichiometry of the outer kinetochore responds to microtubule-proximal regulation
The authors of this manuscript suggest the stoichiometry of outer submodules of the budding yeast kinetochore is strongly influenced by factors at the kinetochore-microtubule interface, including Fin1, Dam1, and microtubule tracking. Outer kinetochore stoichiometry is remarkably plastic and responsi...
ORGANISM(S): Saccharomyces cerevisiae (Baker's yeast) 
2020-01-16 | PXD011384 | Pride
Kinetochore Anaphase Mudpit 
TranscriptomicsMapping of cohesion factor association sites in S. cerevisiae - Meiosis I chromosome segregation is established through regulation of microtubule-kinetochore interactions.
During meiosis, a single round of DNA replication is followed by two consecutive rounds of nuclear divisions called meiosis I and meiosis II. In meiosis I, homologous chromosomes segregate, while sister chromatids remain together. Determining how this unusual chromosome segregation behavior is estab...
ORGANISM(S): Saccharomyces cerevisiae 
2012-10-05 | E-GEOD-41339 | biostudies-arrayexpress
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