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ProteomicsInduced homogenous sporulation by kinA-overexpression leads to altered Bacillus subtilis spore proteomes and spore properties
Summary To facilitate more accurate spore proteomic analysis, the current study focuses on inducing homogeneous sporulation by overexpressing kinA and assesses the effect of sporulation synchronization on spore resistance, structures, the germination behavior at single-spore level and the proteome. ...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-28 | MSV000085346 | MassIVE
TranscriptomicsYeast spore germination: time course
The process of Saccharomyces cerevisiae spore germination includes breakage of dormancy, morphological changes and resumption of vegetative growth. We have determined the global transcriptional response during the first two hours of spore germination in response to rich growth medium and glucose alo...
ORGANISM(S): Saccharomyces cerevisiae 
2012-12-18 | E-GEOD-29960 | biostudies-arrayexpress
ProteomicsIdentification of Native Cross-links in Bacillus subtilis Spore Coat Proteins
The resistance properties of the bacterial spores are partially due to spore surface proteins, ~30% of which are said to form an insoluble protein fraction. Previous research has also identified a group of spore coat proteins affected by spore maturation, which exhibit an increased level of inter pr...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2021-01-09 | MSV000086681 | MassIVE
ProteomicsIntegrative analysis of proteome and transcriptome dynamics during Bacillus subtilis spore revival
Description Bacillus subtilis forms highly resistant, metabolically inactive spores upon nutrient limitation. These endospores pose challenges to the food and medical sectors. Spores reactivate their metabolism upon contact with germinants through germination and outgrowth, and then develop into ve...
ORGANISM(S): Bacillus Subtilis (ncbitaxon:1423) 
2020-04-02 | MSV000085217 | MassIVE
ProteomicsClostridium sordellii outer spore proteins
Bacterial spores play an important role in disease initiation, transmission and persistence. The outermost spore layer, the exosporium, is important as it is the first point of contact between the spore and the environment and may be involved in spore adherence, protection and germination. Clostridi...
ORGANISM(S): Paeniclostridium sordellii ATCC 9714 
2018-05-01 | PXD009235 | Pride
Spore Clostridium sordellii Bacteria 
ProteomicsIdentification of Clostdridium difficile Immunoreactive Spore Proteins of the Epidemic strain R20291
Clostridium difficile infections are the leading cause of diarrhea associated to the use of antibiotics. During infection, C. difficile initiates a sporulation cycle leading to the persistence of C. difficile spores in the host and disease dissemination. Nowadays, the development of vaccine and pass...
ORGANISM(S): Peptoclostridium difficile (strain R20291) (Clostridium difficile) 
2018-07-05 | PXD008592 | Pride
Cdi patients Isoelectric focusing Spores Clostridium difficile Immunoblotting Two-dimensional electrophoresis Serologic reactivity 
ProteomicsCytological and proteomic analyses of horsetail (Equisetum arvense) spore germination
Spermatophyte pollen tubes and root hairs have been used as single-cell-type model systems toward understanding the molecular processes underlying polar growth of plant cells. Horsetail (Equisetum arvense L.) is a perennial herb species in Equisetopsida, which creates separately growing spring and s...
ORGANISM(S): Equisetum arvense (Field horsetail) (Common horsetail) 
2016-12-23 | PXD002218 | Pride
Proteomics Spore germination Single cell Fern Equisetum arvense l. Polar growth 
ProteomicsStoichiometry and absolute abundance of proteins in the Bacillus cereus spore insoluble fraction determined using a QconCAT approach
Spores of Bacillus cereus pose a threat to food safety due to their high resistance to the heat or acid treatments commonly used to make food microbially safe. As spores survive these treatments and later resume growth either on foodstuffs or, after ingestion, upon entering the gut they are capable ...
ORGANISM(S): Bacillus cereus 
2018-10-26 | PXD003569 | Pride
Bacillus cereus Qconcat Quantitative proteomics Spores 
TranscriptomicsLotus rootlets responses to Gigaspora margarita germinating spore exudates (GSE) after 24 and 48 hours
1 mL of 10X Gigaspora margarita spore germinating exudates or water was used to treat two weeks old Lotus rootlets for 24 and 48 hours.
ORGANISM(S): Lotus japonicus 
2015-06-23 | E-MTAB-3119 | biostudies-arrayexpress
ProteomicsCotL, a new morphogenetic coat protein of Clostridium difficile
The strict anaerobe Clostridium difficile is an important nosocomial enteropathogen that has become the most common cause of antibiotic-associated diarrhea. The oxygen-resistant C. difficile spores play a central role in the infectious cycle, contributing to transmission, infection and recurrence. T...
ORGANISM(S): Peptoclostridium difficile (strain 630) (Clostridium difficile) 
2019-10-16 | PXD010359 | Pride
Spore coat Shotgun proteomics Q exactive hf Clostridium difficile 
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