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TranscriptomicsAnalyzing the role of transcription elongation factors Spt4-5 in splicing
A set of 22 expts. aimed at identifying splicing events dependent upon on the Spt4-5 transcription elongation factors in yeast. Four spt mutants and an mRNA capping mutant were analyzed four times each, including biological and technical (dye-swap) replicates. Two wt vs wt expts. were also perform...
ORGANISM(S): Saccharomyces cerevisiae 
2010-06-09 | E-GEOD-1610 | biostudies-arrayexpress
TranscriptomicsThe effects on transcriptome from absence of Spt4/Supt4h by RNA-seq
We have done RNAseq analyses in yeast and in rat and mouse striatal neurons. The experiments in yeast examine globally the physiological effects of spt4 deletion on the transcriptome. The experiments in neurons examine the global effects of supt4 knockdown to a cellular level (i.e., 50% knockdown)...
ORGANISM(S): Rattus norvegicus 
2012-02-15 | E-GEOD-33497 | biostudies-arrayexpress
TranscriptomicsThe DSIF subunits Spt4 and Spt5 have several unexpected, distinct roles in regulating immunoglobulin class switch recombination
Class-switch recombination (CSR), induced by activation-induced cytidine deaminase, can be divided into two phases: DNA cleavage of the switch (S) regions, and the joining of the cleaved ends of the different S regions. Here, we show that the DSIF complex (Spt4 and Spt5), a transcription elongation ...
ORGANISM(S): Mus musculus 
2013-10-22 | E-GEOD-33206 | biostudies-arrayexpress
ProteomicsThe transient Spt4-Spt5 complex as an upstream regulator of non-coding RNAs during development
The Spt4-Spt5 complex is conserved and essential RNA polymerase elongation factor. To investigate the role of the Spt4-Spt5 complex in non-coding transcription during development, we used the unicellular model Paramecium tetraurelia. In this organism harboring both germline (micronucleus - MIC) and ...
ORGANISM(S): Paramecium tetraurelia 
2022-04-27 | PXD027748 | Pride
Interactomics Spt4 Meiosis 
TranscriptomicsExpression data from 10 day old Arabidopsis thaliana seedlings
Microarrays were used to detail the global programme of gene expression comparing wild-type and RNAi knock-down plants of SPT4-1 and SPT4-2 Arabidosis seeds were stratified for 2 days and grown for 10 days on solid (0.8% Agar) MS in an plant incubator in a 16 hours day, 8 hours night rythm. Only th...
ORGANISM(S): Arabidopsis thaliana 
2014-03-07 | E-GEOD-49590 | biostudies-arrayexpress
GenomicsSpt4 promotes Pol I processivity and transcription elongation
Spt4 promotes Pol I processivity and transcription elongation
PRJNA702517 | ENA
GenomicsSpt4 helps RNAPII pass the +2 nucleosome barrier in vivo
Spt4 helps RNAPII pass the +2 nucleosome barrier in vivo
PRJNA668299 | ENA
GenomicsThe effects on transcriptome from absence of Spt4/Supt4h by RNA-seq
The effects on transcriptome from absence of Spt4/Supt4h by RNA-seq
PRJNA148851 | ENA
TranscriptomicsSpt4 promotes Pol I processivity and transcription elongation
The goal of this study was to determine the effect of Spt4 on transcription by Pol I in vivo. Therefore, Native Elongating Transcript Sequencing (NET-seq) was used to evaluate the occupancy of Pol I in wild-type and spt4△ strains. We determined that in spt4△ yeast, there was a processivity defect an...
ORGANISM(S): Saccharomyces cerevisiae 
2021-02-18 | GSE166983 | GEO
GenomicsSpt4 helps RNAPII pass the +2 nucleosome barrier in vivo
Aim and Methods: To understand the precise role of Spt4 in budding yeast, various high throughput sequencing techniques were used. Nucleosome positions were studied using MNase-seq in WT and spt4∆ cells. The position of RNAPII was examined using NET-seq in spt4∆ cells or cells in which Spt4 has been...
ORGANISM(S): Saccharomyces cerevisiae 
2021-09-27 | GSE159291 | GEO
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