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The fidelity of signal transmission requires the binding of regulatory molecules to their cognate targets. However, the crowded cell interior risks off-target interactions between proteins that are functionally unrelated. Understanding the constraints this imposes on cell systems evolution requires ...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae (Baker's yeast) 
2024-09-12 | PXD045466 | Pride
PWWP2A variant NuRD complex represses intragenic spurious transcription initiation
Spurious ASVs
5-hydroxymethylcytosine Promotes Productive Transcription and Inhibits Spurious Promoters
Transient plasmid transfection is common approach for studies in cultured mammalian cells. To examine behavior of transfected plasmids, we analyzed their transcriptional landscape by deep sequencing. We found that plasmids generate different levels of transcripts virtually everywhere. Spurious trans...
ORGANISM(S): Homo sapiens 
H3 trimethylation at K36 deposited over the body of actively transcribed gene, is recognized by readers to modulate the epigenetic states, such as DNA methylation and histone deacetylation, to ensure the proper gene transcription. PWWP2A was identified as the reading component of variant NuRD comple...
ORGANISM(S): Mus musculus 
2020-09-15 | GSE148382 | GEO
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