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ProteomicsOptimization of Phosphotyrosine Proteomics
We optimized a procedure of phosphotyrosine enrichment with immunoprecipitation. Then the optimized procedure was applied into a global analysis of phosphotyrosine status in culture cells treated with Erlotinib.
ORGANISM(S): Homo Sapiens (human) 
2017-09-29 | PXD005061 | JPOST Repository
ProteomicsPeptide probes containing a non-hydrolyzable phosphotyrosine-mimetic residue as a tool for tyrosine phosphatomics
Protein tyrosine phosphatases (PTPs) cooperate with protein tyrosine kinases to regulate intracellular tyrosine phosphorylation levels, and their dysfunction is associated with various diseases. However, due to the low abundance of PTPs in cells, comprehensive analysis requires an enrichment step pr...
ORGANISM(S): Homo Sapiens (human) 
2021-10-27 | PXD025038 | JPOST Repository
ProteomicsMutation-specific and common phosphotyrosine signatures of KRAS G12D and G13D alleles
KRAS is one of the most frequently mutated genes across all cancer subtypes. Two of the most frequent oncogenic KRAS mutations observed in patients result in glycine to aspartic acid substitution at either codon 12 (G12D) or 13 (G13D). Although the biochemical differences between these two predomina...
ORGANISM(S): Homo sapiens (Human) 
2021-06-09 | PXD009843 | Pride
Sw48 Phosphoproteome G13d mutation G12d mutation Kras Signaling 
ProteomicsAutomated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics
Phosphotyrosine (pY) enrichment is critical for expanding fundamental and clinical understanding of cellular signaling by mass spectrometry-based proteomics. However, current pY enrichment methods exhibit a high cost per sample and limited reproducibility due to expensive affinity reagents and manua...
ORGANISM(S): Homo sapiens (Human) 
2023-05-05 | PXD041271 | Pride
Hela Lc-msms Kingfisher Orbitrap eclipse Orbitrap exploris 480 Phosphotyrosine enrichment 
TranscriptomicsThe ShcA PTB Domain Functions as a Biological Sensor of Phospho-tyrosine Signaling During Breast Cancer Progression
The ShcA adaptor possesses two phosphotyrosine binding motifs, which include an SH2 and a PTB domain. In the majority of cases, ShcA utilizes its PTB domain to engage activated receptor tyrosine kinases (RTKs). To establish the mportance of this domain during mammary tumorigenesis, we employed a Sh...
ORGANISM(S): Mus musculus 
2013-08-07 | E-GEOD-41718 | biostudies-arrayexpress
TranscriptomicsPhosphotyrosine-mediated regulation of enterohemorhagic Escherichia coli virulence
Enteric pathogens with low infectious doses rely on the ability to orchestrate expression of virulence and metabolism-associated genes in response to environmental cues for successful infection. Accordingly, the human pathogen enterohemorrhagic Escherichia coli (EHEC) employs a complex multifaceted ...
ORGANISM(S): Escherichia coli O157:H7 str. EDL933 
2018-02-02 | GSE103764 | GEO
ProteomicspY-IP of Colorectal cancer cells
Phosphotyrosine status were compared between colorectal cancer cells which is sensitive or resistant to Cetuximab. Activated kinases in resistant cells were examined as targets to overcome the resistance.
ORGANISM(S): Homo Sapiens (human) 
2017-10-08 | PXD005839 | JPOST Repository
ProteomicsScalable Phosphotyrosine Enrichment with SH2 Superbinder Enables Deep Profiling of EGF Responses
Phosphotyrosine signaling plays a critical role in many biological processes, from cell proliferation to immune response. Despite its importance, proteomic studies of tyrosine phosphorylation have been limited in scale and throughput by costly reagents and labor-intensive protocols. To address this,...
ORGANISM(S): Homo sapiens (Human) Saccharomyces cerevisiae Saccharomyces cerevisiae (Baker's yeast) 
2026-07-03 | PXD062515 | Pride
Phosphotyrosine Automated Hela Enrichment High-throughput Egf Lc-ms/ms 
ProteomicsPhosphotyrosine proteomics of MDCK cell
MDCK cell with Src-overexpression (wild type or mutant) were trypsinized, and enriched with Fe-IMAC. After labelling with TMT 10plex reagent, phosphotyrosine peptides were further enriched with phosphotyrosine antibodies. The enriched peptides were analyzed with Q Exactive Plus.
ORGANISM(S): Homo Sapiens (human) 
2023-03-31 | PXD032878 | JPOST Repository
UnknownRecent advances in synthetic and medicinal chemistry of phosphotyrosine and phosphonate-based phosphotyrosine analogues.
Not available
S-EPMC8130623 | biostudies-literature
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