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ProteomicsCapturing Salmonella SspH2 Host Targets in Virus-Like Particles
In the context of host-pathogen interactions, gram-negative bacterial virulence factors, such as effectors, may be transferred from bacterial to eukaryotic host cytoplasm by multicomponent Type III protein secretion systems (T3SSs). Central to Salmonella enterica serovar Typhimurium (S. Typhimurium)...
ORGANISM(S): Homo sapiens (Human) 
2022-02-17 | PXD026740 | Pride
Virotrap Effector Salmonella Ssph2 
ProteomicsQuantification on NOD1 ubiquitination sites with SspH2 co-overexpression
HEK293T cells were transiently transfected with NOD1-FLAG and HA-SspH2 WT/HA-SspH2 C580/empty vector. Following anti-FLAG automated immunoprecipitation and in-gel trypsin digestion, fractionated samples were injected onto LC-MS/MS. Quantitation was performed on diglycyl-lysine sites on NOD1, on iden...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-24 | MSV000087700 | MassIVE
ProteomicsQuantification on NOD1 ubiquitination sites with ubiquitin and SspH2 co-overexpression
HEK293T cells were transiently transfected with NOD1-FLAG, HA-SspH2 WT/HA-SspH2 C580/empty vector, and Myc-Ubiquitin. Following anti-FLAG automated immunoprecipitation and in-gel trypsin digestion, fractionated samples were injected onto LC-MS/MS. Quantitation was performed on diglycyl-lysine sites ...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2021-06-23 | MSV000087693 | MassIVE
UnknownRecombinant Salmonella expressing SspH2-EscI fusion protein limits its colonization in mice.
Not available
S-EPMC5415771 | biostudies-literature
UnknownThe Salmonella type III effector SspH2 specifically exploits the NLR co-chaperone activity of SGT1 to subvert immunity.
Not available
S-EPMC3723637 | biostudies-literature
UnknownThe <i>Salmonella</i> Effector SspH2 Facilitates Spatially Selective Ubiquitination of NOD1 to Enhance Inflammatory Signaling.
Not available
S-EPMC11412229 | biostudies-literature
UnknownCapturing Salmonella SspH2 Host Targets in Virus-Like Particles.
Not available
S-EPMC8455821 | biostudies-literature
ProteomicsCovalent fragment screening to inhibit the E3 ligase activity of bacterial NEL enzymes SspH1 and SspH2
As the global fight against antimicrobial resistance in bacteria becomes increasingly pressing, new tool compounds are needed to understand and identify novel therapeutic targets. Here, cysteine-directed fragment-based drug discovery is coupled with a high throughput chemistry direct-to-biology scre...
ORGANISM(S): Homo sapiens (Human) 
2025-11-10 | PXD057304 | Pride
Bacterial nels Fragment-based drug discovery Covalent fragments Salmonella effectors Chemoproteomics E3 ligase High throughput chemistry 
ProteomicsGlobal proteomics and ubiquitomics in THP-1 derived macrophages stably expressing SspH2 WT/C580A
THP1 cells stably expressing WT or catalytic mutant C580A SspH2 were differentiated into macrophages, and treated with MG-132 to enrich ubiquitinated proteins. Cells were lysed with probe sonicator in 9 M urea, and proteins were reduced, alkylated, digested with trypsin, and desalted before diGly-mo...
ORGANISM(S): Homo Sapiens (ncbitaxon:9606) 
2025-11-24 | MSV000100008 | MassIVE
UnknownSpecificities and redundancies in the NEL family of bacterial E3 ubiquitin ligases of Salmonella enterica serovar Typhimurium.
Not available
S-EPMC10867120 | biostudies-literature
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