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TranscriptomicsPaternal RNA contributions in the C. elegans zygote
Development of the early embryo is thought to be mainly driven by maternal gene products and post-transcriptional gene regulation. Here, we used metabolic labeling to show that RNA can be transferred by sperm into the embryo. To identify genes with paternal expression in the embryo, we performed cro...
ORGANISM(S): Caenorhabditis elegans 
2014-06-04 | E-GEOD-57351 | biostudies-arrayexpress
TranscriptomicsIllumina sequencing of small RNAs from C. elegans embryos
Caenorhabditis elegans is one of the most prominent model systems to study embryogenesis. However, it has been impractical to collect large amounts of precisely staged embryos. Thus, early C. elegans embryogenesis has not been amenable to most modern high-throughput genomics or biochemistry assays. ...
ORGANISM(S): Caenorhabditis elegans 
2009-09-06 | E-GEOD-17153 | biostudies-arrayexpress
TranscriptomicsGlobal characterization of the oocyte-to-embryo transition in C. elegans uncovers a novel mRNA turnover mechanism
The oocyte-to-embryo transition (OET) is thought to be mainly driven by post-transcriptional gene regulation. However, expression of both RNAs and proteins during the OET has not been comprehensively assayed. Furthermore, specific molecular mechanisms that regulate gene expression during OET are lar...
ORGANISM(S): Caenorhabditis elegans 
2014-07-02 | E-GEOD-58141 | biostudies-arrayexpress
TranscriptomicsIn vivo and transcriptome-wide identification of RNA-binding protein target sites
This SuperSeries is composed of the following subset Series: GSE33569: In vivo and transcriptome-wide identification of RNA-binding protein target sites [PAR-CLIP] GSE33573: In vivo and transcriptome-wide identification of RNA-binding protein target sites [RNA-Seq] Refer to individual Series
ORGANISM(S): Caenorhabditis elegans 
2011-12-09 | E-GEOD-33543 | biostudies-arrayexpress
TranscriptomicsIn vivo and transcriptome-wide identification of RNA-binding protein target sites [RNA-Seq]
Animal mRNAs are regulated by hundreds of RNA binding proteins (RBPs). The identification of RBP targets is crucial for understanding their function. A recent method, PAR-CLIP, uses photoreactive nucleosides to crosslink RBPs to target RNAs in cells prior to immunoprecipitation. Here, we establish i...
ORGANISM(S): Caenorhabditis elegans 
2011-12-09 | E-GEOD-33573 | biostudies-arrayexpress
TranscriptomicsSelect microRNAs are essential for early development in the sea urchin.
microRNAs (miRNAs) are small noncoding RNAs that mediate post-transcriptional gene regulation and have emerged as essential regulators of many developmental events. The transcriptional network during early embryogenesis of the purple sea urchin, Strongylocentrotus purpuratus, is well described and w...
ORGANISM(S): Strongylocentrotus purpuratus 
2012-02-17 | E-GEOD-35876 | biostudies-arrayexpress
TranscriptomicsConservation of mRNA and protein expression during development of C. elegans
Spatiotemporal control of gene expression is crucial for development and subject to evolutionary changes. Although proteins are the final product of most genes, the developmental proteome of an animal has not yet been comprehensively defined, and the correlation between RNA and protein abundance dur...
ORGANISM(S): Caenorhabditis elegans 
2014-01-23 | E-GEOD-53359 | biostudies-arrayexpress
TranscriptomicsIn vivo and transcriptome-wide identification of RNA-binding protein target sites [PAR-CLIP]
Animal mRNAs are regulated by hundreds of RNA binding proteins (RBPs). The identification of RBP targets is crucial for understanding their function. A recent method, PAR-CLIP, uses photoreactive nucleosides to crosslink RBPs to target RNAs in cells prior to immunoprecipitation. Here, we establish i...
ORGANISM(S): Caenorhabditis elegans 
2011-12-09 | E-GEOD-33569 | biostudies-arrayexpress
TranscriptomicsSingle cell 5’ transcript, TCRαβ enriched, and Cell hashing/CITE-seq library preparation on the 10x chromium, and sequencing
Single cells from human colorectal cancer and normal adjacent colon of 16 patients were used for single-cell RNA-seq, TCR-seq, CITE-seq and Cell hashing. In brief, single cells were incubated for 3h with or without PMA/Ionomycin, and were treated with Cell hashing and CITE-seq antibodies to distingu...
ORGANISM(S): Homo sapiens 
2022-03-02 | E-MTAB-9455 | biostudies-arrayexpress
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